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Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures
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Revision as of 04:41, 16 September 2013
Overnight Cultures
Reagents and Materials (per culture prepared)
:10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
5 mL LB
5 μL 1000X antibiotics
Single colonies on a plate (best not to start an overnight from a glycerol stock)
Protocol
Add 3mL sterile/autoclaved LB in a 15mL Falcon tube.
Pipet 3μL of 1000X antibiotic into the LB.
Select a single colony using a sterile toothpick or pipette tip.
Place toothpick or pipette tip in the culture tube and stir.
Remove toothpick, or in the case of a pipette tip, leave in the tube.
Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)
