Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures

From 2013.igem.org

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<h1>Overnight Cultures</h1>
<h1>Overnight Cultures</h1>
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<p><b> Reagents and Materials (per culture prepared)</b></p>:
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<h2> Reagents and Materials (per culture prepared):</h2>
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     <p> 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</p>
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     <li> 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li>
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     <p> 5 mL LB</p>
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     <li> 5 mL LB</li>
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     <p> 5 μL 1000X antibiotics</p>
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     <li> 5 μL 1000X antibiotics</li>
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     <p> Single colonies on a plate (best not to start an overnight from a glycerol stock)</p>
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     <li> Single colonies on a plate (best not to start an overnight from a glycerol stock)</li>
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  <p><b> Protocol</b> </p>
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  <h2>Protocol</h2>
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   <p> Add 3mL sterile/autoclaved LB in a 15mL Falcon tube. </p>
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   <li> Add 3mL sterile/autoclaved LB in a 15mL Falcon tube. </li>
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<p> Pipet 3μL of 1000X antibiotic into the LB.</p>
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<li> Pipet 3μL of 1000X antibiotic into the LB.</li>
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     <p> Select a single colony using a sterile toothpick or pipette tip. </p>
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     <li> Select a single colony using a sterile toothpick or pipette tip. </li>
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     <p> Place toothpick or pipette tip in the culture tube and stir. </p>
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     <li> Place toothpick or pipette tip in the culture tube and stir. </li>
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     <p> Remove toothpick, or in the case of a pipette tip, leave in the tube. </p>
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     <li> Remove toothpick, or in the case of a pipette tip, leave in the tube. </li>
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<p>Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)</p>
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<li>Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)</li>
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Revision as of 04:43, 16 September 2013

Overnight Cultures

Reagents and Materials (per culture prepared):

  • 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
  • 5 mL LB
  • 5 μL 1000X antibiotics
  • Single colonies on a plate (best not to start an overnight from a glycerol stock)

    • Protocol

  • Add 3mL sterile/autoclaved LB in a 15mL Falcon tube.
  • Pipet 3μL of 1000X antibiotic into the LB.
  • Select a single colony using a sterile toothpick or pipette tip.
  • Place toothpick or pipette tip in the culture tube and stir.
  • Remove toothpick, or in the case of a pipette tip, leave in the tube.
  • Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)

    • Retrieved from "http://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures"