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Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA
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<section id="Content"> | <section id="Content"> | ||
<h1>Rehydration of Registry DNA</h1> | <h1>Rehydration of Registry DNA</h1> | ||
| - | + | <p>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used</p> | |
| + | <h2>Reagents and Reagents</h2> | ||
| + | <ul> | ||
| + | <li>Biobrick parts</li> | ||
| + | <li>ddH<sub>2</sub>O (deionized water)</li> | ||
| + | </ul> | ||
<h2>Protocol</h2> | <h2>Protocol</h2> | ||
<ol> | <ol> | ||
| - | |||
<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | <li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | ||
<li>Add 10 μL of ddH<sub>2</sub>O (deionized water)</li> | <li>Add 10 μL of ddH<sub>2</sub>O (deionized water)</li> | ||
Revision as of 01:18, 18 September 2013
Rehydration of Registry DNA
Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used
Reagents and Reagents
- Biobrick parts
- ddH2O (deionized water)
Protocol
- Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
- Add 10 μL of ddH2O (deionized water)
- Let the water incubate at room temperature for 10 minutes
- Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies
