Team:Bielefeld-Germany/Project/Cytochromes

From 2013.igem.org

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==<span style="color:#f88101; padding-left:22px;">'''Cytochromes''' </span>==
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Membranes can work as an isolator for the cell to prevent the transfer of charged molecules. So it is necessary to change the
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membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the
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membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from ''Shewanella oneidensis'' MR-15 should be expressed in ''E. coli''.
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[[Image:Bielefeld-germany-project-overview-cytochromes.png|410x350px|center|MFC overview|]]
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Of great interest is the production of endogenous mediators. The overexpression of glyceroldehydrogenase in ''E. coli'' is a promising approach. Because many derivates of glyceroldehydrogenase are small, water-soluble redoxmolecules, they have the properties of a mediator. Futhermore, it will be tested, if there is a possibility of expressing the mediator phenazin. Phenazin is an endogenous
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Membranes can work as an isolator for the cell to prevent the transfer of charged molecules. So it is necessary to change the
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mediator of ''Pseudomonas'' species.
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membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the
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membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from ''Shewanella oneidensis'' MR-15 should be expressed in ''E. coli''.
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[[Image:placeholder.png|center|thumb|300px|'''Figure 1:''' placeholder]]
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[[Image:Bielefeld-germany-project-overview-cytochromes.png|300px|thumb|center|'''Figure 1:''' Extracellular electron transfer via cytochromes in Shewanella oneidensins MR-1 with a minimal set of genes|]]
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[[Image:Bielefeld-germany-project-overview-cytochromes.pngthumb|center|300px|'''Figure 1:''' Extracellular electron transfer via cytochromes in Shewanella oneidensins MR-1 with a minimal set of genes|]]
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Revision as of 21:17, 18 September 2013





Cytochromes

Membranes can work as an isolator for the cell to prevent the transfer of charged molecules. So it is necessary to change the membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from Shewanella oneidensis MR-15 should be expressed in E. coli.


Bielefeld-germany-project-overview-cytochromes.png







Mediators

Of great interest is the production of endogenous mediators. The overexpression of glyceroldehydrogenase in ''E. coli'' is a promising approach. Because many derivates of glyceroldehydrogenase are small, water-soluble redoxmolecules, they have the properties of a mediator. Futhermore, it will be tested, if there is a possibility of expressing the mediator phenazin. Phenazin is an endogenous mediator of ''Pseudomonas'' species.


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