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| | <title>TzuChiU Formosa</title> | | <title>TzuChiU Formosa</title> |
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| | + | <h3><font size="4" face="calibri"><b>Competent cell</b></font> |
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| + | <font size="3" face="calibri"> |
| - | <h3><b><u>Composing a team</b></u></h3> | + | <ol> |
| | + | <li>Culture DH5-alpha in a glass test tube with 3CC LB at 37°C for 12 hours. |
| | + | <li>Add 200 ul bacterium into a 500cc conical flask with 50 ml LB inside. Culture at 37°C until OD600 reaches 0.2 |
| | + | <li>3. Distribute the bacterium from the conical flask into a 50cc Centrifuge and centrifuge at 4000rpm, 15 minutes at 4 °C |
| | + | <li>Remove supernatant and add in 16 ml CaCl2 (100mM) |
| | + | <li>Centrifuge at 4000rpm, 15 minutes at 4 °C |
| | + | <li>Remove supernatant and add 3 ml CaCl2 (100mM) |
| | + | <li>Centrifuge at 4000rpm, 15 minutes at 4 °C |
| | + | <li>Remove supernatant and add 3 ml CaCl2 (100mM) |
| | + | <li>Place on ice and put in 4 °C cold room for an hour. |
| | + | <li>Cut tip and add 1 ml Glycerol (Takes up 25% of the total volume) |
| | + | <li>Transfer 200 ul into each eppendorf ( This step should be done quickly and taken to -80°C ASAP)<br>* Can use dry ice or ice with alcohol. |
| | + | <li>Plating the next day to test CFU (colony frequency unit) |
| | + | </ol> |
| | + | </font> |
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| - | <img src="https://static.igem.org/mediawiki/2013/5/59/226482_509184505812482_140307941_n.jpg" width="300px" height="190px" align="right" hspace="70px"> | + | </h3> |
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| - | <td><font size="4" face="calibri" vertical-align"top">We were given the opportunity to select members from various faculties to compose our own iGEM team. Qualities of our Hypnoseq member should be adventurous, committed and have a passion for research. </font></td>
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| - | <h3><b><u>Brainstorming</b></u></h3>
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| - | <img src="https://static.igem.org/mediawiki/2013/f/fe/971982_550943391636593_1844422419_n.jpg" width="300px" height="190px" align="right" hspace="70px">
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| - | <td><font size="4" face="calibri" vertical-align"top">After our team was constructed, our members came up with a few possible ideas/ projects for the competition. Basic researches were done to support the ideas for future selection. </font></td>
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| - | <h3><b><u>Team selection</b></u></h3>
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| - | <td><font size="4" face="calibri" vertical-align"top">Our team members then presented the ideas/ projects at our campus iGEM preliminary round. The possible ideas were then assessed and voted by our advisor and peers.</font></td>
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| - | <h3><b><u>Human practice</b></u></h3>
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| - | <img src="https://static.igem.org/mediawiki/2013/1/17/20130719_093506.jpg" width="300px" height="190px" align="right" hspace="70px">
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| - | <td><font size="4" face="calibri" vertical-align"top">Out team members went to a few junior high schools to promote synthetic biology to the scholars. Surveys were also handed out after the presentation so we have an overall perception of their understanding of synthetic biology.</font></td>
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| - | <h3><b><u>Launching Wiki</b></u></h3>
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| - | <img src="https://static.igem.org/mediawiki/2013/0/05/Slide_picture_5.png" width="300px" height="190px" align="right" hspace="70px">
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| - | <td><font size="4" face="calibri" vertical-align"top">After our direction was set and our goal was clear, we started to construct our iGEM webpage, filling in related details and keeping our records up to date.</font></td>
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| - | <h3><b><u>Kick start to our experiment</b></u></h3>
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| - | <td><font size="4" face="calibri" vertical-align"top">In order to save time, we did some basic preparations for the experiment before all the parts were received. Preparations include primer design, preparing LB plates, making competent cells etc. so when the biobrick is received the actual experiment may start immediately. </font></td>
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