Team:TU-Munich/Notebook/Materials

From 2013.igem.org

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<H2>Reagents</H2>
<H2>Reagents</H2>
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<H3>Chemicals</H3>
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{|cellspacing="0" border="1"
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|Name
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|Supplier
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Revision as of 23:32, 15 June 2013



Reagents

Chemicals

Name Supplier


Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3'
TEV_rv 5'- atataaccggtattcatgagttgagtcgcttcc - 3'
TEV_t387c_fw 5'- atggtgtcagacactagctgcacattcccttcatc - 3'
TEV_t387c_rv 5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3'
N_split_TEV_fw 5'- attcgcggccgcttctag - 3'
N_split_TEV_rv 5'- ttcgaaaccggtagtttggaagttggttgtcac - 3'
C_split_TEV_fw 5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3'
C_split_TEV_rv 5'- ccgctactagtattaaccgg - 3'
PIF6_2-100_fw 5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3'
PIF6_2-100_rv 5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3'
MinMCS_fo 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3'
MinMCS_rev 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- cacttaagcattttctacgggtcgatcacaccattca - 3'
Bpu_SDMI_rev 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3'
Bpu_SDMII_fo 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3'
Bpu_SDMII_re 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3'
Bpu_SDMIII_fo 5'- cacccttaaaaataaagctggctgcggacaggaag - 3'
Bpu_SDMIII_re 5'- cttcctgtccgcagccagctttatttttaagggtg - 3'
Bpu_SDMIV_fo 5'- cgtacgctcactcttacgggtacacaggataaata - 3'
Bpu_SDMIV_re 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'

Instruments

Elektrophoresis

Photometers

Nanodrop Spektrohpotometer


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