Team:TU-Munich/Notebook/Methods

From 2013.igem.org

(Difference between revisions)
(Physcomitrella Methods)
Line 33: Line 33:
==Physcomitrella Methods==
==Physcomitrella Methods==
 +
The techniques used for the cultivation and manipulation of ''Physcomitrella patens'' are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible under [[http://www.plant-biotech.net Plant-Biotech.net]]
===Preparation of Knob-Media===
===Preparation of Knob-Media===

Revision as of 23:57, 15 June 2013


Molecular-biological Methods

Preparation of Nucleotide fragments

Digestion, Dephosphorylation

Hybridization of Oligonucleotides

Polymerase Chain Reaction

Site-directed Mutagenesis

Agarose Gel Electrophoresis

Ligation of DNA-Fragments

Microbiological Methods

Preparation of LB-Media

Transformation of E. coli

Cultivation of E. coli

Protein-biochemical Methods

Physcomitrella Methods

The techniques used for the cultivation and manipulation of Physcomitrella patens are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible under http://www.plant-biotech.net Plant-Biotech.net

Preparation of Knob-Media

Cultivation of P. patens

Homogenization

Bioinformatical Methods

$(document).ready(function(){


// put the footer in the right place

$("#footer-box").prepend($("#social-footer"));


// implement image preloading

var images = new Array()

function preload() {

   for (i = 0; i < preload.arguments.length; i++) {
       images[i] = new Image()
       images[i].src = preload.arguments[i]
   }

}


// preload menu backgrounds

preload( "TUM13_menu-highlight.png",

        "TUM13_submenu-bg.png",
        "TUM13_submenu-highlight.png",
        "TUM13_tour-start-highlight.png",
        "TUM13_tour-previous-highlight.png",
        "TUM13_tour-next-highlight.png" );

// preload team pictures

if ( $("div#teamfield").length > 0 ) { preload( "TUM13_raven-front.png",

        "TUM13_raven-top.png",
        "TUM13_raven-top-left.png",
        "TUM13_raven-left.png",
        "TUM13_raven-bottom-left.png",
        "TUM13_raven-bottom.png",
        "TUM13_raven-bottom-right.png",
        "TUM13_raven-right.png",
        "TUM13_raven-top-right.png",
        "TUM13_katrin-front.png",        // Katrin
        "TUM13_katrin-t.png",
        "TUM13_katrin-t-l.png",
        "TUM13_katrin-l.png",
        "TUM13_katrin-b-l.png",
        "TUM13_katrin-b.png",
        "TUM13_katrin-b-r.png",
        "TUM13_katrin-r.png",
        "TUM13_katrin-t-r.png",
        "TUM13_rosario-front.png",        // Rosario
        "TUM13_rosario-t.png",
        "TUM13_rosario-t-l.png",
        "TUM13_rosario-l.png",
        "TUM13_rosario-b-l.png",
        "TUM13_rosario-b.png",
        "TUM13_rosario-b-r.png",
        "TUM13_rosario-r.png",
        "TUM13_rosario-t-r.png",
        "TUM13_fabian-front.png",        // Fabian
        "TUM13_fabian-t.png",
        "TUM13_fabian-t-l.png",
        "TUM13_fabian-l.png",
        "TUM13_fabian-b-l.png",
        "TUM13_fabian-b.png",
        "TUM13_fabian-b-r.png",
        "TUM13_fabian-r.png",
        "TUM13_fabian-t-r.png",
        "TUM13_andreas-front.png",        // Andreas
        "TUM13_andreas-t.png",
        "TUM13_andreas-t-l.png",
        "TUM13_andreas-l.png",
        "TUM13_andreas-b-l.png",
        "TUM13_andreas-b.png",
        "TUM13_andreas-b-r.png",
        "TUM13_andreas-r.png",
        "TUM13_andreas-t-r.png",
        "TUM13_louise-front.png",        // Louise
        "TUM13_louise-t.png",
        "TUM13_louise-t-l.png",
        "TUM13_louise-l.png",
        "TUM13_louise-b-l.png",
        "TUM13_louise-b.png",
        "TUM13_louise-b-r.png",
        "TUM13_louise-r.png",
        "TUM13_louise-t-r.png",
        "TUM13_johanna-front.png",        // Johanna
        "TUM13_johanna-t.png",
        "TUM13_johanna-t-l.png",
        "TUM13_johanna-l.png",
        "TUM13_johanna-b-l.png",
        "TUM13_johanna-b.png",
        "TUM13_johanna-b-r.png",
        "TUM13_johanna-r.png",
        "TUM13_johanna-t-r.png",
        "TUM13_meike-front.png",        // Meike
        "TUM13_meike-t.png",
        "TUM13_meike-t-l.png",
        "TUM13_meike-l.png",
        "TUM13_meike-b-l.png",
        "TUM13_meike-b.png",
        "TUM13_meike-b-r.png",
        "TUM13_meike-r.png",
        "TUM13_meike-t-r.png",
        "TUM13_volker-front.png",        // Volker
        "TUM13_volker-t.png",
        "TUM13_volker-t-l.png",
        "TUM13_volker-l.png",
        "TUM13_volker-b-l.png",
        "TUM13_volker-b.png",
        "TUM13_volker-b-r.png",
        "TUM13_volker-r.png",
        "TUM13_volker-t-r.png",
        "TUM13_polte-front.png",        // Polte
        "TUM13_polte-t.png",
        "TUM13_polte-t-l.png",
        "TUM13_polte-l.png",
        "TUM13_polte-b-l.png",
        "TUM13_polte-b.png",
        "TUM13_polte-b-r.png",
        "TUM13_polte-r.png",
        "TUM13_polte-t-r.png",
        "TUM13_leonie-front.png",        // Leonie
        "TUM13_leonie-t.png",
        "TUM13_leonie-t-l.png",
        "TUM13_leonie-l.png",
        "TUM13_leonie-b-l.png",
        "TUM13_leonie-b.png",
        "TUM13_leonie-b-r.png",
        "TUM13_leonie-r.png",
        "TUM13_leonie-t-r.png",
        "TUM13_philipp-front.png",        // Philipp
        "TUM13_philipp-t.png",
        "TUM13_philipp-t-l.png",
        "TUM13_philipp-l.png",
        "TUM13_philipp-b-l.png",
        "TUM13_philipp-b.png",
        "TUM13_philipp-b-r.png",
        "TUM13_philipp-r.png",
        "TUM13_philipp-t-r.png",
        "TUM13_jeff-front.png",        // Jeff
        "TUM13_jeff-t.png",
        "TUM13_jeff-t-l.png",
        "TUM13_jeff-l.png",
        "TUM13_jeff-b-l.png",
        "TUM13_jeff-b.png",
        "TUM13_jeff-b-r.png",
        "TUM13_jeff-r.png",
        "TUM13_jeff-t-r.png",
        "TUM13_chris-front.png",        // Chris
        "TUM13_chris-t.png",
        "TUM13_chris-t-l.png",
        "TUM13_chris-l.png",
        "TUM13_chris-b-l.png",
        "TUM13_chris-b.png",
        "TUM13_chris-b-r.png",
        "TUM13_chris-r.png",
        "TUM13_chris-t-r.png",
        "TUM13_flo-front.png",        // Flo
        "TUM13_flo-t.png",
        "TUM13_flo-t-l.png",
        "TUM13_flo-l.png",
        "TUM13_flo-b-l.png",
        "TUM13_flo-b.png",
        "TUM13_flo-b-r.png",
        "TUM13_flo-r.png",
        "TUM13_flo-t-r.png" );

}

// Slideshows

$('.bxslider').bxSlider({

   responsive: false,
   auto: true,
   autoHover: true

});

$('.bxgallery').bxSlider({

   slideMargin: 10,
   minSlides: 3,
   maxSlides: 3,
   moveSlides: 1,
   slideWidth: 5000

});

$("ul.bxgallery img").slimbox({ loop: true }, function(el) { url = el.src; url = url.substring(0, url.lastIndexOf('/')); url = url.replace('/thumb/', '/'); // description = $(el).parents("div.thumbinner").children("div.thumbcaption").text(); return [url, ]; }, function(el) { return (this == el) || (this.parentNode.parentNode && (this.parentNode.parentNode == el.parentNode.parentNode)); });


// Counter and Countdown

function render_counter(c) { i = 0; iid = window.setInterval(function(){ if ( (c-i) > (c/200) ) { $('span#counter').html(i); i += Math.round(c/200); } else { $('span#counter').html(c); window.clearInterval(iid); } }, 10); }

if ($('span#counter').length > 0) { $.ajax({ url: "https://2013.igem.org/Special:PopularPages", success: function( html ) { dom = $.parseHTML(html); visitors = $(dom).find('a[title="Team:TU-Munich"]').parent().text(); visitors = visitors.substring(visitors.indexOf('(')+1); visitors = visitors.substring(0, visitors.indexOf(' ')); visitors = visitors.replace(',', ); render_counter(visitors); }, error: function( xhr, status ) { render_counter(4700); } }); }

if ($('span#countdown) { clock = window.setInterval(function(){ jetzt = new Date(); time_left = Date.UTC(2013, 9, 5, 4, 0, 0) - Date.UTC(jetzt.getUTCFullYear(), jetzt.getUTCMonth(), jetzt.getUTCDate(), jetzt.getUTCHours(), jetzt.getUTCMinutes(), jetzt.getUTCSeconds()); left_sec = (time_left/1000)%60; left_sec = (left_sec < 10) ? "0" + left_sec : left_sec; left_min = Math.floor(time_left/60000)%60; left_min = (left_min < 10) ? "0" + left_min : left_min; left_h = Math.floor(time_left/3600000)%24; left_h = (left_h < 10) ? "0" + left_h : left_h; left_d = Math.floor(time_left/86400000); left_d = (left_d == 1) ? left_d + " day" : left_d + " days"; $('span#countdown').html(left_d + " " + left_h + ":" + left_min + ":" + left_sec); }, 1000); }

// Animate teamfield

if ( $("div#teamfield").length > 0 ) {

var $members = $("div#teamfield a");

$("body").mousemove(function(event){ for (i=0; i<$members.length; i++) {

if ( $members.eq(i).offset().left > event.pageX ) {

   if ( $members.eq(i).offset().top > event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("top-left");
   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("left");
   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom-left");
   }

} else if ( $members.eq(i).offset().left <= event.pageX && ( $members.eq(i).offset().left + $members.eq(i).width() ) >= event.pageX ) {

   if ( $members.eq(i).offset().top > event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("top");
   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("front");
   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom");
   }    

} else if ( ( $members.eq(i).offset().left + $members.eq(i).width() ) < event.pageX ) {

   if ( $members.eq(i).offset().top > event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("top-right");
   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("right");
   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {
       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom-right");
   }  

}

} });

}

});