Team:Paris Saclay/Notebook/August/23

From 2013.igem.org

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===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
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(((((((((((((((===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
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===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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We can see anything. The digestion did work.
We can see anything. The digestion did work.
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{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Revision as of 20:53, 23 September 2013

Contents

Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3

1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI

]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 5µL of Bba_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
  • Gel : 1%

Expect sizes :

  • PSB3K3 : 2750 bp

We can see anything. The digestion did work.