Team:Calgary/Notebook/Protocols/GlycerolStocks
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Revision as of 06:53, 24 September 2013
Glycerol Stocks
Glycerol Stocks
Reagents and Materials
- Overnight bacterial growth
- Screw Cap Tubes
- Glycerol
Protocol
- Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes
- Add 0.5mL of overnight culture to each tube
- Pipet up and down to gently mix
- Place one tube in -20°C freezer
- Place the other tube in -80°C freezer