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Team:Calgary/Notebook/Protocols/VerificationDigest
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Revision as of 07:04, 24 September 2013
Verification Digest
Verification Digest
Reagents and Materials
- Restriction enzymes
- Appropriate buffer
- Plasmid purified DNA
- 1% agarose gel
Protocol
- In a tube, add (per reaction):
- 4µL Plasmid purified DNA
- 1µL appropriate buffer
- 0.2µL enzyme 1
- 0.2µL enzyme 2
- 3.6µL ddH2O
- Incubate at 37 degrees Celsius for 1h
- Run 10µL in a 1% agarose gel at 100V
