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Team:Calgary/Notebook/Protocols/VerificationDigest
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<li>Appropriate buffer</li> | <li>Appropriate buffer</li> | ||
<li>Plasmid purified DNA</li> | <li>Plasmid purified DNA</li> | ||
| - | <li>1% <a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AgaroseGelElectrophoresis">agarose gel</a></li> | + | <li>1% <a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AgaroseGelElectrophoresis" target="_blank">agarose gel</a></li> |
</ul> | </ul> | ||
<h2>Protocol</h2> | <h2>Protocol</h2> | ||
Revision as of 07:05, 24 September 2013
Verification Digest
Verification Digest
Reagents and Materials
- Restriction enzymes
- Appropriate buffer
- Plasmid purified DNA
- 1% agarose gel
Protocol
- In a tube, add (per reaction):
- 4µL Plasmid purified DNA
- 1µL appropriate buffer
- 0.2µL enzyme 1
- 0.2µL enzyme 2
- 3.6µL ddH2O
- Incubate at 37 degrees Celsius for 1h
- Run 10µL in a 1% agarose gel at 100V
