"
Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples
From 2013.igem.org
(Difference between revisions)
Wkeithvan (Talk | contribs)
(Created page with "<html> <div id="Banner"><h1>Glass Beads Cell Lysis Protocol</h1></div> </html> {{Team:Calgary/ContentPage}} <html> <section id="Content"> <h1>Glass Beads Cell Lysis Protocol for ...")
Newer edit →
(Created page with "<html> <div id="Banner"><h1>Glass Beads Cell Lysis Protocol</h1></div> </html> {{Team:Calgary/ContentPage}} <html> <section id="Content"> <h1>Glass Beads Cell Lysis Protocol for ...")
Newer edit →
Revision as of 07:07, 24 September 2013
Glass Beads Cell Lysis Protocol
Glass Beads Cell Lysis Protocol for Protein Samples
Reagents and Materials
- 0.1m diameter glass beads
- Lysis Buffer
- Overnight induced cultures
- 1.5mL tubes
Protocol
- Culture and induce protein-producing bacteria
- Centrifuge cells at max for 10 minutes. Discard supernatant
- Resuspend cells in 2mL of lysis buffer
- In a 1.5mL tube, add ~0.5mL of glass beads and 1mL of cells resuspended in lysis buffer
- Leave on ice for 5 min. Leave on BeadBeater or Vortex for 5 min. Repeat this cycle 6 times
- Centrifuge at max for 20 minutes
- Transfer supernatant (crude lysate) to a new tube and discard the glass beads
