In the iGEM, most teams have inserted their Biobrick parts or devices into the plasmids and use them. It is true that there are many good points to use plasmids, for instance, it is easy to do transformation, and it is convenient to use high copy plasmid when you want to get high gene expression amount. However, there are some bad points to use plasmids too. For example, when the reproduction starting point of plural plasmids are covered, it can’t put in E.coli at the same time. Also it is difficult to control genetic expression closely.

Therefore, in this year, our team tried to standardize a new method to inserted Biobrick parts or devices in a genome of E.coli and used them. Also we really inserted the device which we designed in a genome of E.coli according to our method and functionalized it.