"
Team:CU-Boulder/Notebook/Experiments
From 2013.igem.org
(Difference between revisions)
| Line 98: | Line 98: | ||
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 - Gel Separation of Proteins</a> <a href="https://static.igem.org/mediawiki/2013/1/1f/Gel_Separation_of_Proteins.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 - Gel Separation of Proteins</a> <a href="https://static.igem.org/mediawiki/2013/1/1f/Gel_Separation_of_Proteins.pdf"><i class="icon-download"></i></a></dt> | ||
<dd>In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.</dd> | <dd>In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/RTXexperiment">Experiment 15.1 - Protein Purification Using RTX</a> <a href="https://static.igem.org/mediawiki/2013/a/a1/RTXExperiment_%281%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment tested the efficacy of RTX as a protein purification method.</dd> | ||
</dl> | </dl> | ||
</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 20:32, 25 September 2013
- Experiment 1.1 - Chemical Transformation Efficiency
- This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
- Experiment 2.1 - Competent Cell Efficiency
- In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.
- Experiment 2.2 - Transformation Recovery Period
- We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.
- Experiment 2.3 - Transformation Recovery Medium
- In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.
- Experiment 3.1 - Promega Miniprep Protocol
- Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.
- Experiment 5.1 - Calculating Competent Cell Efficiency
- We calculated the efficiency of our competent cells using iGEM's transformation efficiency kit.
- Experiment 6.1 - Competent Cell Efficiency
- This experiment was conducted to compare the relative efficiency of a new batch of competent cells to our original supply.
- Experiment 6.2 - Gel Separation of Proteins Methods
- In this experiment we tested different ways to separate RFP with an agarose gel.
- Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment
- This experiment tests the effectiveness of each of our homemade miniprep buffers individually within the Qiagen miniprep system.
- Experiment 11.1 - Mini-column Recycling Protocol
- This experiment was conducted to test whether our mini-column recycling protocol was truly effective at removing residual DNA from used miniprep mini-columns.
- Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers
- Here we tested our homemade miniprep buffers against the commercial Qiagen buffers as a complete set.
- Experiment 11.3 - Mini-column Recycling Protocol Pt. II
- This experiment was designed to compare the effectiveness of recycled mini-columns to new mini-columns.
- Experiment 12.1 - Calculating Competent Cell Efficiency
- Here we tested the efficiency of our most recent batch of competent cells using iGEM's tranformation efficiency kit.
- Experiment 14.1 - Gel Separation of Proteins
- In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.
- Experiment 15.1 - Protein Purification Using RTX
- This experiment tested the efficacy of RTX as a protein purification method.
