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Team:Calgary/Notebook/Protocols/PrussianBlueFerritinSynthesis
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<h1>Prussian Blue Ferritin Synthesis</h1> | <h1>Prussian Blue Ferritin Synthesis</h1> | ||
| + | <h2> Reagents and Materials </h2> | ||
| + | <li>Horse Spleen Ferritin (47mg/mL) </li> | ||
| + | <li>Hydrogen Chloride (pH 3.0)</li> | ||
| + | <li> K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M) </li> | ||
| + | <li> Sodium acetate-acetic acid buffer (pH 3.6)</li> | ||
| + | <li> </li> | ||
| + | |||
| + | <p> 20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.</p> | ||
| + | <p> After the 12 hour period, 5mL of the solution was dialyzed against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 24 hours, with the buffer being replaced after 8 hours. | ||
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Revision as of 17:13, 26 September 2013
Synthesis
Prussian Blue Ferritin Synthesis
Reagents and Materials
20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K4Fe(CN)6 (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.
After the 12 hour period, 5mL of the solution was dialyzed against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 24 hours, with the buffer being replaced after 8 hours.
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