Team:ZJU-China/Notebook/LabNotes/May

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Revision as of 03:14, 27 September 2013

Lab Notes

May June July August September

Protocol

Brainstorm

The "Elf"

Background Design Results Perspetives

Lab Notes: May

Date	Notes
May 24	First lab day! Calibrate the pipettes, and check the working status of PCR machines. Made a plan for wet lab work.
May 25	Set up new LB+Ch1 culture plates. BBa_K411003 culture. Sterilization of culture plates, cuvettes, centrifuge tubes, and PCR tubes.
May 26	PCR amplification of BBa_K411003.
May 27	Cut BBa_K411003 and pSB1c3 with EcoR I and Pst I.
May 28	Gel electrophoresis of the PCR products on May 27th. Failed because of inadequate operations.
May 29	Collect the products of PCR on May 28th. Cut BBa_K411003 with E+P and transform.
May 30	Find out that pSB1c3 and pK411003 colonies are in good condition. Pick up colonies of these parts and transform again.
May 31	Gel electrophoresis to analyze the results of transformation --- failed. Plasmid purification of pSB1c3.

@@ Line 16: / Line 16: @@
 !Notes
 |-
-|May 24th||First lab day! Calibrate the pipettes, and check the working status of PCR machines. Made a plan for wet lab work.
+|May 24||First lab day! Calibrate the pipettes, and check the working status of PCR machines. Made a plan for wet lab work.
 |-
-|May 25th||Set up new LB+Ch1 culture plates. BBa_K411003 culture. Sterilization of culture plates, cuvettes, centrifuge tubes, and PCR tubes.
+|May 25||Set up new LB+Ch1 culture plates. BBa_K411003 culture. Sterilization of culture plates, cuvettes, centrifuge tubes, and PCR tubes.
 |-
-|May 26th||PCR amplification of BBa_K411003.
+|May 26||PCR amplification of BBa_K411003.
 |-
-|May 27th||Cut BBa_K411003 and pSB1c3 with EcoR I and Pst I.
+|May 27||Cut BBa_K411003 and pSB1c3 with EcoR I and Pst I.
 |-
-|May 28th||Gel electrophoresis of the PCR products on May 27th. Failed because of inadequate operations.
+|May 28||Gel electrophoresis of the PCR products on May 27th. Failed because of inadequate operations.
 |-
-|May 29th||Collect the products of PCR on May 28th. Cut BBa_K411003 with E+P and transform.
+|May 29||Collect the products of PCR on May 28th. Cut BBa_K411003 with E+P and transform.
 |-
-|May 30th||Find out that pSB1c3 and pK411003 colonies are in good condition. Pick up colonies of these parts and transform again.
+|May 30||Find out that pSB1c3 and pK411003 colonies are in good condition. Pick up colonies of these parts and transform again.
 |-
-|May 31st||Gel electrophoresis to analyze the results of transformation --- failed. Plasmid purification of pSB1c3.
+|May 31||Gel electrophoresis to analyze the results of transformation --- failed. Plasmid purification of pSB1c3.
 |}