Team:ZJU-China/Notebook/LabNotes/June

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Revision as of 03:16, 27 September 2013

Lab Notes

May June July August September

Protocol

Brainstorm

The "Elf"

Background Design Results Perspetives

Lab Notes: June

Date	Notes
June 1	Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P.
June 2	Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP).
June 3	Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time.
June 4	Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st.
June 5	Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010.
June 6	Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.
June 7	Plasmid purification of 23L part. Store strains in -80°C.
June 8	Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP.
June 9	Small-scale plasmid purification of GFP.
June 10
June 11	Preliminary experiment using new competent cell of DH52, BL21, and ???????
June 12	Pick up colonies of GFP and R0010 and cultivate in LB+A medium.
June 13-30	Final examinations for the semester.

@@ Line 13: / Line 13: @@
 {|
 |-
-!width="80px"|Date
+!width="65px"|Date
 !Notes
 |-
-|June 1st||Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P.
+|June 1||Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P.
 |-
-|June 2nd||Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP).
+|June 2||Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP).
 |-
-|June 3rd||Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time.
+|June 3||Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time.
 |-
-|June 4th||Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st.
+|June 4||Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st.
 |-
-|June 5th||Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010.
+|June 5||Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010.
 |-
-|June 6th||Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.
+|June 6||Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.
 |-
-|June 7th||Plasmid purification of 23L part. Store strains in -80°C.
+|June 7||Plasmid purification of 23L part. Store strains in -80°C.
 |-
-|June 8th||Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP.
+|June 8||Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP.
 |-
-|June 9th||Small-scale plasmid purification of GFP.
+|June 9||Small-scale plasmid purification of GFP.
 |-
-|June 10th||
+|June 10||
 |-
-|June 11th||Preliminary experiment using new competent cell of DH52, BL21, and ???????
+|June 11||Preliminary experiment using new competent cell of DH52, BL21, and ???????
 |-
-|June 12th||Pick up colonies of GFP and R0010 and cultivate in LB+A medium.
+|June 12||Pick up colonies of GFP and R0010 and cultivate in LB+A medium.
 |-
-|June 13th-30th||Final examinations for the semester.
+|June 13-30||Final examinations for the semester.
 |}