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Team:ZJU-China/Notebook/LabNotes/June
From 2013.igem.org
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|June 10|| | |June 10|| | ||
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| - | |June 11||Preliminary experiment using new competent cell of DH52 | + | |June 11||Preliminary experiment using new competent cell of DH52, and BL21. |
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|June 12||Pick up colonies of GFP and R0010 and cultivate in LB+A medium. | |June 12||Pick up colonies of GFP and R0010 and cultivate in LB+A medium. | ||
Revision as of 03:17, 27 September 2013
Lab Notes: June
| Date | Notes |
|---|---|
| June 1 | Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P. |
| June 2 | Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP). |
| June 3 | Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time. |
| June 4 | Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st. |
| June 5 | Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010. |
| June 6 | Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes. |
| June 7 | Plasmid purification of 23L part. Store strains in -80°C. |
| June 8 | Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP. |
| June 9 | Small-scale plasmid purification of GFP. |
| June 10 | |
| June 11 | Preliminary experiment using new competent cell of DH52, and BL21. |
| June 12 | Pick up colonies of GFP and R0010 and cultivate in LB+A medium. |
| June 13-30 | Final examinations for the semester. |
