"
Team:Calgary/Notebook/Protocols/MiniExpression
From 2013.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
<html> | <html> | ||
<section id="Content"> | <section id="Content"> | ||
| - | < | + | <h2>Mini Expression</h2> |
<p class="noIndent">We used this protocol to define the optimum conditions for expression of our constructs.</p> | <p class="noIndent">We used this protocol to define the optimum conditions for expression of our constructs.</p> | ||
Latest revision as of 09:03, 27 September 2013
Mini Expression
Mini Expression
We used this protocol to define the optimum conditions for expression of our constructs.
Reagents and Materials
- Overnight cultures of protein-producing bacteria
- LB broth
- IPTG (Isopropyl-Beta-D-Thiogalactoside)
Protocol
- Make 2mL overnight cultures of your protein-producing bacteria
- Subculture in 10mL of LB broth
- When OD600 is between 0.3 and 0.5, induce the culture with IPTG
- To determine optimum conditions for expression, use different time point of induction (e.g. 4h, 8h and 12h) and different concentration of IPTG (e.g., 0.1mM, 0.4mM and 0.8mM)
