Team:OUC-China

From 2013.igem.org

(Difference between revisions)
(Undo revision 202186 by PengYong (talk))
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<html lang="en">
<html lang="en">
<head>
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
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<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
+
<link href="http://oucast.com/igem/css/bootstrap.css" rel="stylesheet" media="screen">
 +
<link href="http://oucast.com/igem/css/bootstrap-responsive.css" rel="stylesheet" media="screen">
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 +
 
<style>
<style>
#top-section{
#top-section{
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     background-color:transparent;
     background-color:transparent;
     overflow:hide;
     overflow:hide;
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}
}
#p-logo{display:none;}
#p-logo{display:none;}
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}
}
#content h1.firstHeading {display: none;}
#content h1.firstHeading {display: none;}
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#content
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{
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border:none;
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height: 100%;
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margin:0;
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padding:0;
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width:100%;
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position: absolute;
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background-color: #FFFFFF;
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}
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 +
{
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margin:0;
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padding:0;
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}
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li {
 +
      font-weight:normal;
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      font-size:12px;
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}
 +
 +
#menubar {
 +
    background-color:transparent;
 +
    position: relative;
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    float:left;
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    white-space: nowrap;
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    top:-6px;
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    width: 490px;
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    font-family: sans-serif;
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    font-size: 10%;
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    line-height: 1em;
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    z-index:99;
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}
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/* GLOBAL STYLES
 +
    -------------------------------------------------- */
 +
    /* Padding below the footer and lighter body text */
 +
 +
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    h1 {
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color:#0099FF;
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}
 +
    body {
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      padding-bottom: 40px;
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      color: #5a5a5a;
 +
  background: #e5e5e5 url(image/moon.png);
 +
  overflow-x: hidden;
 +
  -webkit-user-select: none;
 +
  -webkit-tap-highlight-color: rgba(0,0,0,0);
 +
    }
 +
 +
    .jumbotron {
 +
        margin: 60px 0;
 +
        text-align: center;
 +
      }
 +
      .jumbotron h1 {
 +
        font-size: 72px;
 +
        line-height: 1;
 +
      }
 +
      .jumbotron .btn {
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        font-size: 21px;
 +
        padding: 14px 24px;
 +
      }
 +
 +
      /* Supporting marketing content */
 +
      .marketing {
 +
        margin: 60px 0;
 +
      }
 +
      .marketing p + h4 {
 +
        margin-top: 28px;
 +
      }
 +
 +
    /* CUSTOMIZE THE NAVBAR
 +
    -------------------------------------------------- */
 +
 +
    /* Special class on .container surrounding .navbar, used for positioning it into place. */
 +
    .navbar-wrapper {
 +
      position: absolute;
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      top: 0;
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      left: 0;
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      right: 0;
 +
      z-index: 10;
 +
      margin-top: 20px;
 +
      margin-bottom: -90px; /* Negative margin to pull up carousel. 90px is roughly margins and height of navbar. */
 +
    }
 +
    .navbar-wrapper .navbar {
 +
 +
    }
 +
 +
    /* Remove border and change up box shadow for more contrast */
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    .navbar .navbar-inner {
 +
      border: 0;
 +
      -webkit-box-shadow: 0 2px 20px rgba(10,0,0,.25);
 +
        -moz-box-shadow: 0 2px 20px rgba(10,0,0,.25);
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              box-shadow: 0 2px 20px rgba(10,0,0,.25);
 +
    }
 +
 +
    /* Downsize the brand/project name a bit */
 +
    .navbar .brand {
 +
      padding: 14px 20px 16px; /* Increase vertical padding to match navbar links */
 +
      font-size: 16px;
 +
      font-weight: bold;
 +
      text-shadow: 0 -1px 0 rgba(0,0,0,.5);
 +
    }
 +
 +
    /* Navbar links: increase padding for taller navbar */
 +
    .navbar .nav > li > a {
 +
      padding: 15px 20px;
 +
    }
 +
 +
    /* Offset the responsive button for proper vertical alignment */
 +
    .navbar .btn-navbar {
 +
      margin-top: 10px;
 +
    }
 +
 +
 +
 +
    /* CUSTOMIZE THE CAROUSEL
 +
    -------------------------------------------------- */
 +
     /* Carousel base class */
     /* Carousel base class */
     .carousel {
     .carousel {
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       margin-top: 10px;
       margin-top: 10px;
     }
     }
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 +
 +
     /* MARKETING CONTENT
     /* MARKETING CONTENT
     -------------------------------------------------- */
     -------------------------------------------------- */
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       margin-right: 60px;
       margin-right: 60px;
     }
     }
 +
 +
     /* Featurettes
     /* Featurettes
     ------------------------- */
     ------------------------- */
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       letter-spacing: -1px;
       letter-spacing: -1px;
     }
     }
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 +
     /* RESPONSIVE CSS
     /* RESPONSIVE CSS
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     }
     }
-
/*the nav settings
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/* = 通用 Global
----------------------------------------------- */
----------------------------------------------- */
-
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
+
a:not(.btn) {
-
color: #1abc9c;
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color: #00699E;
-
background-color: transparent;
+
-webkit-transition: 300ms;
 +
-moz-transition: 300ms;
 +
transition: 300ms;
}
}
-
.navbar-inverse .navbar-nav>li>a{
+
a:hover:not(.btn),
-
color: #fff;
+
a:active:not(.btn) {
 +
color: #9E0000;
 +
text-decoration: none;
 +
-webkit-transform: translateY(-8px);
 +
-moz-transform: translateY(-8px);
 +
transform: translateY(-8px);
}
}
-
.navbar-inverse .navbar-nav>li>a:hover{
+
 
-
color: #1abc9c;
+
hr.soften {
-
background-color:transparent;
+
height: 1px;
 +
margin: 30px 0;
 +
background-image: -webkit-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
 +
background-image:   -moz-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
 +
background-image:     -ms-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
 +
background-image:      -o-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
 +
border: 0;
}
}
-
.navbar-inverse .navbar-brand{
+
 
-
          color: #fff;
+
 
-
          padding: 0;
+
.nav a {
 +
-webkit-transition: 500ms;
 +
-moz-transition: 500ms;
 +
transition: 500ms;
}
}
-
.navbar-inverse .navbar-brand img{
+
header {
-
  height: 50px;
+
margin-top: 40px;
-
  padding-left: 90px;
+
}
}
-
.navbar-inverse .navbar-brand p{
+
.hero-unit {
-
  padding:15px 30px;
+
background: url(/images/cover-5.jpg);
-
  margin:0;
+
background-size: cover;
 +
filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='/images/cover-5.jpg', sizingMethod='scale');
 +
-ms-filter: "progid:DXImageTransform.Microsoft.AlphaImageLoader(src='/images/cover-5.jpg', sizingMethod='scale')";
 +
color: #fff;
 +
height: 100px;
 +
margin-bottom: 0;
}
}
-
.navbar-inverse{
+
.hero-unit h1 {
-
border-color: transparent;
+
/* font: 88px Minion,'Optima', "Times New Roman", Times, serif; */
-
background: #34495e;
+
text-align: center;
-
font-weight: bold;
+
text-shadow: 1px 1px 10px black;
-
filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
+
}
}
-
.dropdown-menu{
+
.hero-unit img {
-
background-color:#34495e;
+
max-width: 500px;
-
border:none;
+
-webkit-animation: title-in 1s ease-out 0 1 forwards;
-
-webkit-box-shadow:none;
+
-webkit-transition: 500ms;
-
box-shadow: none;
+
-moz-transition: 500ms;
-
}
+
transition: 500ms;
-
.dropdown-menu>li>a{
+
 
-
color: #fff;
+
-
font-weight: bold;
+
}
}
-
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
+
.hero-unit img:hover {
-
color:#1abc9c;
+
-webkit-transform: translateY(-5px) scale(1.05) rotate(2deg);
-
background-color: transparent;
+
-moz-transform: translateY(-5px) scale(1.05) rotate(2deg);
 +
transform: translateY(-5px) scale(1.05) rotate(2deg);
}
}
-
/*add dropdown-submenu to btV3*/
+
</style>
-
.dropdown-submenu{position:relative;}
+
-
.dropdown-submenu>.dropdown-menu{top:0;left:100%;margin-top:-6px;margin-left:-1px;-webkit-border-radius:0 6px 6px 6px;-moz-border-radius:0 6px 6px 6px;border-radius:0 6px 6px 6px;}
+
-
.dropdown-submenu:hover>.dropdown-menu{display:block;}
+
-
.dropdown-submenu>a:after{display:block;content:" ";float:right;width:0;height:0;border-color:transparent;border-style:solid;border-width:5px 0 5px 5px;border-left-color:#cccccc;margin-top:5px;margin-right:-10px;}
+
-
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
+
-
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
+
-
</style>
+
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
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</head>
</head>
<body >
<body >
-
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
+
 
-
<div class="navbar-header">
+
 
-
<button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
+
 
-
<span class="sr-only">The navigation</span>
+
<div class="navbar navbar-inverse navbar-fixed-top">
-
      <span class="icon-bar"></span>
+
      <div class="navbar-inner">
-
      <span class="icon-bar"></span>
+
        <div class="container">
-
      <span class="icon-bar"></span>
+
<div class="row"><div class="span3"><a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2013/6/6a/Ouc-igem1.jpg" alt="logo" style="width:70px;height:50px;margin-top:0px;padding:0 0 0 0;" class="brand png"></a></div>
-
      </button>
+
          <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
-
    <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
+
            <span class="icon-bar"></span>
-
      <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
+
            <span class="icon-bar"></span>
-
    </a>
+
            <span class="icon-bar"></span>
-
    <a class="navbar-brand" href="https://2013.igem.org/Team:OUC-China">
+
          </button>
-
      <p>OUC-China IGEM 2013</p>
+
            <div class="nav-collapse collapse">
-
    </a>
+
                  <ul class="nav pull-right">
-
  </div>
+
  <li class="active"><a href="https://2013.igem.org/Team:OUC-China"><font size="4.5px">Home</font></a></li>
-
    <div class="collapse navbar-collapse navbar-ex1-collapse">
+
              <li class="dropdown">
-
    <ul class="nav navbar-nav pull-right">
+
                <a class="dropdown-toggle" id="drop4" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Project</font><b class="caret"></b>
-
<li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
+
</a>
-
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
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                <ul id="menu1" class="dropdown-menu" role="menu" aria-labelledby="drop4">
-
            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
+
<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a>
-
            <ul class="dropdown-menu">
+
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a>
-
<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
+
                  <li class="dropdown-submenu">
-
                <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
+
                  <a tabindex="-1" href="#">Intercellular compartment</a>
-
                  <li class="dropdown-submenu">
+
  <ul class="dropdown-menu">
-
                  <a tabindex="-1" href="#">Intercellular compartment</a>
+
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
-
  <ul class="dropdown-menu">
+
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
+
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
+
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
+
 
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
+
  </ul>
-
  </ul>
+
  </li>
-
  </li>
+
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
 +
 
 +
  </ul>
  </ul>
  </li>
  </li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
 +
                  <li role="presentation" class="divider"></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
                 </ul>
                 </ul>
-
          </li>
+
              </li>
-
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
+
              <li class="dropdown">
-
            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
+
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Modeling</font><b class="caret"></b></a>
-
                 <ul class="dropdown-menu">
+
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
 +
                  <li role="presentation" class="divider"></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                 </ul>
                 </ul>
-
            </li>
+
              </li>
-
            <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
+
              <li class="dropdown">
-
<li class="dropdown">
+
                <a  href="https://2013.igem.org/Team:OUC-China/Human Practice"><font size="4.5px">Human Practice</font></a>
-
            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
+
               
-
            <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
+
              </li>
 +
<li class="dropdown">
 +
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Team</font><b class="caret"></b></a>
 +
              <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
-
</ul>
+
  </ul>
-
            </li>
+
              </li>
-
<li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
+
  <li class="dropdown">
-
<li class="dropdown">
+
                <a  href="https://2013.igem.org/Team:OUC-China/Safety"><font size="4.5px">Safety</font></a>
-
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
+
               
-
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
+
              </li>
 +
  <li class="dropdown">
 +
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Revision as of 09:22, 27 September 2013

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Abstract

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

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Specialist of our project

1.We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.
2.We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.
3.We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.
4.We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in High oxygen partial pressure environment.

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Reconstructing the Magnetosome Membrane in E. coli

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