Team:INSA Toulouse/contenu/lab practice/notebook/calendar/general inducer

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  <h2 class="faq" title="Click to open the FAQ">June 2013</h2>
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  <div class="infos">
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  <ul class="circlearrow">
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  <li><span class="spantitle2">Week 3 (24-30 June)</span></br>
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Amplification of new biobricks for cloning: <br>
 +
BBa_J23119: Strong promoter,<br>
 +
BBa_P0440: rbs tetR term,<br>
 +
BBa_I13521: Ptet rbs RFP term.</li>
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  </ul>
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</li>>
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  </div>
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</div>
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<div class="clear"></div>
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<div class="accordeon">
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  <h2 class="faq" title="Click to open the FAQ">July 2013</h2>
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  <div class="infos">
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  <ul class="circlearrow">
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  <li><span class="spantitle2">Week 4 (1-7 July)</span><br>
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Assembly of a strong promoter with tetR. tetR protein is necessary to inhibit the Ptet promoter.<br>
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  <img src="https://static.igem.org/mediawiki/2013/e/ef/General_inducer_-_h140px.png" class="imgcontent" /></li>
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  <li><span class="spantitle2">Week 5 (8-14 July)</span><br>
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Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.<br>
 +
  <img src="https://static.igem.org/mediawiki/2013/b/b2/General_inducer2_-_h140px.png" class="imgcontent" /><br>
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The construction is then sent for sequencing.<br><br>
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</li>
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  <li><span class="spantitle2">Week 6 (15-21 July)</span><br>
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The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.<br>
 +
  <img src="https://static.igem.org/mediawiki/2013/2/2f/General_inducer3_-_h140px.png" class="imgcontent" />
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</li>
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  <li><span class="spantitle2">Week 7 (22-28 July)</span><br>
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We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.<br><br>
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</li>
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  </div>
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</div>
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<div class="clear"></div>
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<div class="accordeon">
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  <h2 class="faq" title="Click to open the FAQ">August 2013</h2>
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  <div class="infos">
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  <ul class="circlearrow">
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  <li><a href="">August 2013</a>
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  <ul class="circlearrow">
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  <li><span class="spantitle2">Week 8 (29-4 August)</span><br>
 +
The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP.<br><br></li>
 +
  <li><span class="spantitle2">Week 9 (5-11 August)</span><br>
 +
Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.<br>
 +
  <img src="https://static.igem.org/mediawiki/2013/1/11/General_inducer4_-_h190px.png" class="imgcontent" /></li>
 +
  <li><span class="spantitle2">Week 10 (12-18 August)</span><br>
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None of the passed assemblies worked, so we tried with a weak promoter.<br>
 +
  <img src="https://static.igem.org/mediawiki/2013/5/59/General_inducer5_-_h140px.png" class="imgcontent" /><br>
 +
This one seems to work but we are waiting for the sequencing to confirm the construct.<br><br></li>
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  <li><span class="spantitle2">Week 11 (19-25 August) </span><br>
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</li>
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  </div>
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</div>
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<div class="clear"></div>
   <div class="list2">
   <div class="list2">

Revision as of 10:40, 27 September 2013

logo


Calendar

General Inducer Characterization

June 2013

  • Week 3 (24-30 June)
    Amplification of new biobricks for cloning:
    BBa_J23119: Strong promoter,
    BBa_P0440: rbs tetR term,
    BBa_I13521: Ptet rbs RFP term.
>

July 2013

  • Week 4 (1-7 July)
    Assembly of a strong promoter with tetR. tetR protein is necessary to inhibit the Ptet promoter.
  • Week 5 (8-14 July)
    Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.

    The construction is then sent for sequencing.

  • Week 6 (15-21 July)
    The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.
  • Week 7 (22-28 July)
    We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.

August 2013

  • August 2013
    • Week 8 (29-4 August)
      The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP.

    • Week 9 (5-11 August)
      Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.
    • Week 10 (12-18 August)
      None of the passed assemblies worked, so we tried with a weak promoter.

      This one seems to work but we are waiting for the sequencing to confirm the construct.

    • Week 11 (19-25 August)
  • June 2013
    • Week 3 (24-30 June)
      Amplification of new biobricks for cloning:
      BBa_J23119: Strong promoter,
      BBa_P0440: rbs tetR term,
      BBa_I13521: Ptet rbs RFP term.
  • July 2013
    • Week 4 (1-7 July)
      Assembly of a strong promoter with tetR. tetR protein is necessary to inhibit the Ptet promoter.
    • Week 5 (8-14 July)
      Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.

      The construction is then sent for sequencing.

    • Week 6 (15-21 July)
      The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.
    • Week 7 (22-28 July)
      We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.

  • August 2013
    • Week 8 (29-4 August)
      The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP.

    • Week 9 (5-11 August)
      Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.
    • Week 10 (12-18 August)
      None of the passed assemblies worked, so we tried with a weak promoter.

      This one seems to work but we are waiting for the sequencing to confirm the construct.

    • Week 11 (19-25 August)


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