Team:USTC CHINA/Project/Results
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<img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="125"/> | <img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="125"/> | ||
<p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b, which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP. </p> | <p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b, which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP. </p> | ||
- | <img src="https://static.igem.org/mediawiki/2013/d/d7/2013ustc-chinajiaotuTD1-GFP.jpg"></br></br> | + | <div class="atfigure" align="center"><img src="https://static.igem.org/mediawiki/2013/d/d7/2013ustc-chinajiaotuTD1-GFP.jpg"></br></br> |
- | + | Fig1. SDS PAGE shows the molecule weight of TD1-GFP</div> | |
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<div style="float:left;width:290px;"> | <div style="float:left;width:290px;"> |
Revision as of 11:48, 27 September 2013