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Team:TMU-Tokyo/Parts
From 2013.igem.org
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<h1>Parts data</h1> | <h1>Parts data</h1> | ||
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<li><a href="http://parts.igem.org/Part:BBa_K1015013">BBa_K1015013―Km-Blunt end </a><br> | <li><a href="http://parts.igem.org/Part:BBa_K1015013">BBa_K1015013―Km-Blunt end </a><br> | ||
This is what connected the blunt end to cohesive end of pSB1C3.</li> | This is what connected the blunt end to cohesive end of pSB1C3.</li> | ||
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<li><a href="http://parts.igem.org/Part:BBa_K1015014">BBa_K1015014―IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)</a><br> | <li><a href="http://parts.igem.org/Part:BBa_K1015014">BBa_K1015014―IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)</a><br> | ||
This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. | This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. | ||
</li> | </li> | ||
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<li><a href="http://parts.igem.org/Part:BBa_K1015015">BBa_K1015015―araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)</a><br> | <li><a href="http://parts.igem.org/Part:BBa_K1015015">BBa_K1015015―araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)</a><br> | ||
With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.</li> | With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.</li> | ||
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<li><a href="http://parts.igem.org/Part:BBa_K1015016">BBa_K1015016―lacI+FRT+Cm</a><br> | <li><a href="http://parts.igem.org/Part:BBa_K1015016">BBa_K1015016―lacI+FRT+Cm</a><br> | ||
This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). | This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). | ||
</li> | </li> | ||
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<li><a href="http://parts.igem.org/Part:BBa_K1015017">BBa_K1015017:FRT+Ap(for RED system)</a><br> | <li><a href="http://parts.igem.org/Part:BBa_K1015017">BBa_K1015017:FRT+Ap(for RED system)</a><br> | ||
This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out. </li> | This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out. </li> | ||
Revision as of 13:34, 27 September 2013
Submitted parts
Parts data
- BBa_K1015013―Km-Blunt end
This is what connected the blunt end to cohesive end of pSB1C3. - BBa_K1015014―IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. - BBa_K1015015―araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)
With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD. - BBa_K1015016―lacI+FRT+Cm
This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). - BBa_K1015017:FRT+Ap(for RED system)
This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out.
