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Team:TMU-Tokyo/Notebook experiment/3rd
From 2013.igem.org
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<h1>Experiment > 3rd week</h1> | <h1>Experiment > 3rd week</h1> | ||
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| + | <Br> | ||
<b>September 15th</b><Br> | <b>September 15th</b><Br> | ||
| + | PCR result<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/6/65/TMU-Tokyo_0915.jpg"><Br> | ||
| + | 1: Fragment 1, 2: Fragment 2, 3: Fragment 3 - Ap,<Br> | ||
| + | 4: Fragment 3- Cm, 5: pBAD-fimE<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | Ligation fragment and vector. | ||
| + | <div style="text-align:center;"> | ||
| + | <Table cellpadding="5"> | ||
| + | <Tr> | ||
| + | <Td>Plasmid name</Td><Td>Fragment 1-A+B</Td><Td>Fragment 2</Td><Td>Fragment 3 - Ap</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Fragment</Td><Td>Fragment 1-A<Br>Fragment 1-B</Td><Td>Fragment 2</Td><Td>Fragment 3 - Ap</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Vector</Td><Td>pSB1C3</Td><Td>pSB1C3</Td><Td>pSB1C3</Td> | ||
| + | </Tr> | ||
| + | </Table> | ||
| + | </div> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | Transformation following plasmid. | ||
| + | <Table><Tr><Td>Fragment 1, 2, 3 - Ap</Td></Tr></Table> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | |||
<b>September 16th</b><Br> | <b>September 16th</b><Br> | ||
| + | Mini prep following plasmid. | ||
| + | <Table><Tr><Td>Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt</Td></Tr></Table> | ||
| + | Result<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/4/44/TMU-Tokyo_0916.jpg"><Br> | ||
| + | 1: Fragment 1, 2: Fragment 3 - Ap,<Br> | ||
| + | 3-5: Fragment 3 - Cm, 6-8: Km-Blunt<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | |||
<b>September 17th</b><Br> | <b>September 17th</b><Br> | ||
Revision as of 20:07, 27 September 2013
Experiment > 3rd week
September 15th
PCR result
1: Fragment 1, 2: Fragment 2, 3: Fragment 3 - Ap,
4: Fragment 3- Cm, 5: pBAD-fimE
Ligation fragment and vector.
| Plasmid name | Fragment 1-A+B | Fragment 2 | Fragment 3 - Ap |
| Fragment | Fragment 1-A Fragment 1-B | Fragment 2 | Fragment 3 - Ap |
| Vector | pSB1C3 | pSB1C3 | pSB1C3 |
Transformation following plasmid.
| Fragment 1, 2, 3 - Ap |
September 16th
Mini prep following plasmid.
| Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt |
1: Fragment 1, 2: Fragment 3 - Ap,
3-5: Fragment 3 - Cm, 6-8: Km-Blunt
September 17th
September 18th
September 19th
September 20th
September 21th
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