Team:Calgary/Notebook/Protocols/OvernightCultures

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<h2> Reagents and Materials (per culture prepared):</h2>
<h2> Reagents and Materials (per culture prepared):</h2>
<ul>
<ul>
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<li>10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li>
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<li>10mL culture tube (16mm x 160mm or 16mm x 125mm) or 15mL Falcon tube</li>
<li>5mL LB</li>
<li>5mL LB</li>
<li>5μL 1000X antibiotics</li>
<li>5μL 1000X antibiotics</li>

Revision as of 20:47, 27 September 2013

Overnight Cultures

Reagents and Materials (per culture prepared):

  • 10mL culture tube (16mm x 160mm or 16mm x 125mm) or 15mL Falcon tube
  • 5mL LB
  • 5μL 1000X antibiotics
  • Single colonies on a plate (best not to start an overnight from a glycerol stock)

Protocol

  1. Add 3mL sterile/autoclaved LB in a 15mL Falcon tube
  2. Pipet 3μL of 1000X antibiotic into the LB
  3. Select a single colony using a sterile toothpick or pipette tip
  4. Place toothpick or pipette tip in the culture tube and stir
  5. Remove toothpick, or in the case of a pipette tip, leave in the tube
  6. Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)

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