Team:CU-Boulder/Project/Kit/DNAPurification

From 2013.igem.org

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<dt>Homemade Miniprep System</dt>
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<dd>We have developed a homemade miniprep system that is much cheaper that the commercial plasmid purification kits. In addition, we have developed an effective method of recycling mini-columns to reduce waste!</dd>
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<dd> We designed a method for making our own minipreps that is much cheaper than commercial plasmid purification kits. Additionally, we tested and validated an effective method of recycling mini-columns to reduce both cost and waste!</dd>
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Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulted from contaminating plasmid from the previous purification.  Recycling works.
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Revision as of 23:30, 27 September 2013

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Homemade Miniprep System

We designed a method for making our own minipreps that is much cheaper than commercial plasmid purification kits. Additionally, we tested and validated an effective method of recycling mini-columns to reduce both cost and waste!

Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulted from contaminating plasmid from the previous purification. Recycling works.

Gel Purification of DNA

This simple procedure utilizes filter paper and dialysis tubing to obtain concentrated DNA from an agarose gel, eliminating the need for commercial gel purification kits.

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