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Team:Calgary/Project/Achievements
From 2013.igem.org
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<ul><li>Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in <i> E. coli </i> by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for <i> E. coli</i> and added a his-6 tag such that this protein can be purified using affinity chromatography.</li> | <ul><li>Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in <i> E. coli </i> by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for <i> E. coli</i> and added a his-6 tag such that this protein can be purified using affinity chromatography.</li> | ||
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| + | <h2>For our <b>Human Practices</b> we...</h2> | ||
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| + | <h2>For our <b>Informed Design</b> we...</h2> | ||
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| + | <li> We used Informed Design: We designed our project with the cattle industries culture and needs first and foremost. </li> | ||
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| + | <li> Our project began with extensive consultation with key cattle industry stakeholders who explained the industries culture and how our technology could best fit into this it. We spoke with a rancher (Bob Church), feedlot operator (Chinook Feeders), a veterinarian (Feedlot Management Services), a meat processor (Cargill), and vaccine producer (Bioniche). </li> | ||
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| + | <li> We continued to consult with these industry stakeholders throughout the development of our FerriTALE system to ensure our technology would continue to meet the needs of the industry. Their industrial experience helped us to at many points during the summer redesign and pivot our system to better fit within the industry.</li> | ||
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| + | <h2>For our <b>Safety</b> we...</h2> | ||
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| + | <li> Safety was a paramount concern with FerriTALES construction and use. We developed them as an in vitro protein based system that significantly reduced the risk of an organism escaping. The FerriTALE detection system is produced with engineered bacteria, but only the proteins required to form the FerriTALE are used in the test strip system.</li> | ||
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| + | <li> Assessment of Accuracy: False positives and negatives could have a profound effect on the economics of FerriTALEs use. We have included both a positive and negative controls in our system to reduce these possible impacts.</li> | ||
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| + | <h2>For our <b>Collaboration</b> we...</h2> | ||
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| + | <li> We collaborated with the Paris Bettencourt iGEM Team in developing a database of biosensors made in the iGEM competitions.</li> | ||
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| + | <h2>For our <b>Parts</b> we...</h2> | ||
</section> | </section> | ||
</html> | </html> | ||
Revision as of 02:34, 28 September 2013
Achievements
Achievements
For our Sensor we...
- Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in E. coli by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for E. coli and added a his-6 tag such that this protein can be purified using affinity chromatography.
- We submitted two novel reporters: beta-lactamase and ferritin. We showed that these enzymes
- We characterized beta-lactamase as a colorimetric reporter using phenol red. We also showed that beta
-
For our Human Practices we...
For our Informed Design we...
- We used Informed Design: We designed our project with the cattle industries culture and needs first and foremost.
- Our project began with extensive consultation with key cattle industry stakeholders who explained the industries culture and how our technology could best fit into this it. We spoke with a rancher (Bob Church), feedlot operator (Chinook Feeders), a veterinarian (Feedlot Management Services), a meat processor (Cargill), and vaccine producer (Bioniche).
- We continued to consult with these industry stakeholders throughout the development of our FerriTALE system to ensure our technology would continue to meet the needs of the industry. Their industrial experience helped us to at many points during the summer redesign and pivot our system to better fit within the industry.
- Safety was a paramount concern with FerriTALES construction and use. We developed them as an in vitro protein based system that significantly reduced the risk of an organism escaping. The FerriTALE detection system is produced with engineered bacteria, but only the proteins required to form the FerriTALE are used in the test strip system.
- Assessment of Accuracy: False positives and negatives could have a profound effect on the economics of FerriTALEs use. We have included both a positive and negative controls in our system to reduce these possible impacts.
- We collaborated with the Paris Bettencourt iGEM Team in developing a database of biosensors made in the iGEM competitions.
