Team:Paris Saclay/Notebook/August/23

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===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
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===='''1 - Digestion of Bba_J04450 by EcoRI/PstI'''====
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===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of Bba_J04450 by EcoRI/PstI'''====
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XioaJing
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Used quantities :
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* Buffer FD: 2µL
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* H2O : 5µL
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* DNA : 9µL
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* EcoRI FD : 1µL
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* PstI FD : 1µL
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We let the digestion at 37°C during 10 minutes.
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===='''2 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
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Revision as of 18:28, 28 September 2013

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Contents

Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3

1 - Electrophoresis to check the ethanol precipitation of the digestion of Bba_J04450 by EcoRI/PstI

]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 5µL of Bba_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
  • Gel : 1%

Expect sizes :

  • PSB3K3 : 2750 bp

We can see anything. The digestion did work.


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