Notebook : june 24

Lab work

- Order of oligonucleotides and strains

- Research the intergenic sequence which contain the promoter for pseudomonas pseudoalcaligenes KF 707

- Research on rhodococcus rhodoccrous RHA1

- Research on Registry of standard biological parts

- Search and document about the BioBrick which contain the lacZ with RBS and terminator

- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM.

- Designing the Fnr promoter primers called pfrn_ up and pfnr_down for respectively the forward and the reverse

- Listing the BioBricks which we may have a need

- Planning the first part manipulation (the first and second BBa)

- Control and verification if we have some BioBrick with RBS and Terminators that could be used after

- General presentation about PCBs for Wiki written in Word

- 3 ml of culture of E.Coli DH5α in LB prepared for overnight culture at 17h