Team:TU-Delft/Protocol 3

From 2013.igem.org

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<h2 align="center">Making glycerol stocks</h2>
<h2 align="center">Making glycerol stocks</h2>
<h4 align="left">Requirements:</h4>
<h4 align="left">Requirements:</h4>
-
1. Glycerol
+
<ol>
-
2. Pipettes
+
<li> Glycerol</li>
-
3. 1.5 mL Tubes
+
<li> Pipettes</li>
-
4.     Culture Sample
+
<li> 1.5 mL Tubes</li>
 +
<li>     Culture Sample</li>
 +
</ol>
<br>
<br>
<h4 align="left">Prodecure:</h4>
<h4 align="left">Prodecure:</h4>
-
1. Label the tubes and add 180μL of glycerol. <br>
+
<ol>
-
2. Pipette out 1000 μL of the culture. <br>
+
<li> Label the tubes and add 180μL of glycerol. </li>
-
3. Add the culture to the tubes and store in -80°C<br>
+
<li> Pipette out 1000 μL of the culture. </li>
 +
<li> Add the culture to the tubes and store in -80°C</li>
<br>
<br>
The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.  
The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.  

Revision as of 12:21, 29 September 2013



Protocols

Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.


Making glycerol stocks

Requirements:

  1. Glycerol
  2. Pipettes
  3. 1.5 mL Tubes
  4. Culture Sample

Prodecure:

  1. Label the tubes and add 180μL of glycerol.
  2. Pipette out 1000 μL of the culture.
  3. Add the culture to the tubes and store in -80°C

    4. The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.

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