Team:Evry/Notebook/w16

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<h2 align="center"> 4<sup>th</sup> October </h2>
<h2 align="center"> 4<sup>th</sup> October </h2>
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<h2>Microfluidic experiment</h2>
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<p><b>30/09/13</b><br/>
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Chip's channel were full of bacteria, even after 2 hours of washing <i>(with free iron M9)</i> because we let the bacteria growth over the week-end <i>(in LB medium)</i>. Hence, we prepared another chip to launch another experiment on the next morning.<br/>
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As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.<br/>
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The first results appear promising, but we need to confirm them with a proper experiment.
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  <a title="Bacteria expressing sfGFP, 1st experiment" href="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg">
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    <img alt="Bacteria expressing sfGFP, 1st experiment" src="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg" class="Picture"/>
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    <b>Figure 1:</b> <i>Escherichia coli</i> (TOP 10 strain) expressing sfGFP.<br/>
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                    At the top of the picture, we can see that the channel is full of bacteria.
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Revision as of 21:38, 30 September 2013

Iron coli project

Week 16: 30th September - 6th October

4th October

Microfluidic experiment

30/09/13
Chip's channel were full of bacteria, even after 2 hours of washing (with free iron M9) because we let the bacteria growth over the week-end (in LB medium). Hence, we prepared another chip to launch another experiment on the next morning.
As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.
The first results appear promising, but we need to confirm them with a proper experiment.

Bacteria expressing sfGFP, 1st experiment
Figure 1: Escherichia coli (TOP 10 strain) expressing sfGFP.
At the top of the picture, we can see that the channel is full of bacteria.

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