Biosafety

Motivation

Biosafety is an essential aspect when taking part in iGEM especially when you work with living organisms which could possibly get out of your application by damage or incorrect handling. It is important to protect the population and the environment by developing and applying systems which comply these aspects. In order to counter this problem there exist useful systems to prevent the bacteria from escaping or killing the bacteria when they are outside of the application. To complement this archive we constructed not only one system but also three systems which differ in leakiness and strength. For this approach we combined two common Biosafety-ideas, an auxotrophic and a toxic gene product, in one device. So the constructed Biosafety-System takes the best of this two approaches and is characterized by a double kill-swtich system. This double kill-switch mechanism provides additional a higher plasmid stability and a higher resistance towards undesirable mutations. In one sentence: Our Biosafety-System is safe!

Application

The system we designed is everywhere applicable. Because of the fact that our system is so save and is unindependent of weather it can be used in wastewater treatment, for small applications at home or in the Third World. For detailed information see our Application site.

References

• Autoren (Jahr) Titel [Link|Paper Ausgabe: Seiten].