Data Page

Overview

This page gives a basic overview about our our project Ecolectricity - currently available. A detailed description of our subprojects can be found in our project part of the Wiki.

Project description: The goal of our project is to generate electrical energy with a genetically modified Escherichia coli in a self-constructed fuel cell. Besides the design, construction and technical optimization of the fuel cell, we investigate different genetic approaches. Specific electron transfer proteins have been compiled from a variety of organisms, in order to gain an Escherichia coli Fuel Cell platform, which turns E. coli to an electro active organism. The main challenge is due to an efficient electron transfer from the bacteria to the electrode. Therefore we facilitate and improve electron donation by producing electron-shuttles, so called endogenous mediators, as well as permeabilizing the cell surface by integrating large membrane porins and providing a direct electron pathway by conductive transmembrane protein structures. All these electron transport elements increase electron transfer and bioelectricity generation.

Data for our favorite new parts

1. <bbpart>BBa_K1172501</bbpart> - OprF – Outer membrane porin from Pseudomonas fluorescens: This outer membrane porin enhances membrane permeability and electron shuttle-mediated extracellular electron transfer for higher bioelectricity output.
2. <bbpart>BBa_K1172901</bbpart> - Alanin-Racemase from E. coli: Short part description.
3. <bbpart>BBa_K1172303</bbpart> - Riboflavin synthesis gene cluster from Shewanella oneidensis: Short part description.
4. <bbpart>BBa_K1172909</bbpart> - Biosafety-System S (AraC): Short part description.

We have also characterized the following parts (Coding)

1. <bbpart>BBa_K1172201</bbpart> - GldA - Glycerol dehydrogenase from Escherichia coli: Glycerol dehydrogenase can be used for endogenous mediator production of NADH. Higher amount of electron shuttles improve transfer of electrons from bacteria to the anode and makes the MFC more efficient.
2. <bbpart>BBa_K1172301</bbpart> - NorM: Na+ Antiporter from Shewanella oneidensis: Short part description.
3. <bbpart>BBa_K1172302</bbpart> - RibC: 6,7-dimethyl-8-ribityllumazine synthase alpha subunit RibC from Shewanella oneidensis: Short part description.
4. <bbpart>BBa_K1172401</bbpart> - MtrCAB – Cytochromes from Shewanella oneidensis: Short part description.
5. <bbpart>BBa_K1172904</bbpart> - Rnase Ba (Barnase) from Bacillus Amyloliquefaciens: Short part description.
6. <bbpart>BBa_K1172916</bbpart> - Double plac-Promoter: Short part description.

We have also characterized the following parts (Devices)

1. <bbpart>BBa_K1172203</bbpart> - gldA with T7 promoter and strong RBS: This glycerol dehydrogenase device is used to characterize the endogenous mediator production of NADH and seems to be the best part for efficient NADH production.
2. <bbpart>BBa_K1172204</bbpart> - gldA with IPTG inducible lacI promoter and strong RBS: This glycerol dehydrogenase device is used to characterize the endogenous mediator production of NADH.
3. <bbpart>BBa_K1172205</bbpart> - gldA with medium Anderson promoter and medium RBS: This glycerol dehydrogenase device is used to characterize the endogenous mediator production of NADH.

1. <bbpart>BBa_K1172502</bbpart> - oprF with T7 Promoter and strong RBS: This device is used to characterize the outer membrane porin expression and its effect on the cell surface permeability. This part is the most efficient OprF expression device leading to most improved bioelectricity output.
2. <bbpart>BBa_K1172503</bbpart> - oprF with IPTG inducible lacI promoter and strong RBS: This device is used to characterize the outer membrane porin expression and its effect on the cell surface permeability.
3. <bbpart>BBa_K1172504</bbpart> - oprF with medium Anderson promoter and weak RBS: This device is used to characterize the outer membrane porin expression and its effect on the cell surface permeability.
4. <bbpart>BBa_K1172505</bbpart> - oprF with medium Anderson promoter and medium RBS: This device is used to characterize the outer membrane porin expression and its effect on the cell surface permeability.
5. <bbpart>BBa_K1172507</bbpart> - oprF with strong Anderson promoter and strong RBS: This device is used to characterize the outer membrane porin expression and its effect on the cell surface permeability.

1. <bbpart>BBa_K1172304</bbpart> - Riboflavin synthesis gene cluster from Shewanella oneidensis with T7 promotor and strong RBS: Short part description.
2. <bbpart>BBa_K1172305</bbpart> - Riboflavin synthesis gene cluster from Shewanella oneidensis with medium Anderson promoter and medium RBS: Short part description.
3. <bbpart>BBa_K1172306</bbpart> - Riboflavin synthesis gene cluster from Shewanella oneidensis with strong Anderson promoter and strong RBS: Short part description.

1. <bbpart>BBa_K1172402</bbpart> - mtrCAB gene cluster with IPTG inducible lacI promoter and RBS: Short part description.
2. <bbpart>BBa_K1172403</bbpart> - mtrCAB with medium promoter and medium RBS: Short part description.
3. <bbpart>BBa_K1172404</bbpart> - mtrCAB with strong promoter and strong RBS: Short part description.
4. <bbpart>BBa_K1172405</bbpart> - mtrCAB with T7 promoter and strong RBS: Short part description.

1. <bbpart>BBa_K1172902</bbpart> - Alanin-Racemase (alr) under the control of the ptac-promoter: Short part description.
2. <bbpart>BBa_K1172903</bbpart> - Alanine-Racemase (alr) with double terminator: Short part description.
3. <bbpart>BBa_K1172905</bbpart> - Part 1 of the Biosafety- System S (pRha AraC alr): Short part description.
4. <bbpart>BBa_K1172906</bbpart> - Part 2 of the Biosafety-System S (pBAD Barnase): Short part description.
5. <bbpart>BBa_K1172908</bbpart> - Part 1 of the Biosafety-System L (pRha lacI alr): Short part description.
6. <bbpart>BBa_K1172910</bbpart> - Part 2 of the Biosafety-System L with Barnase (dplac Barnase): Short part description.
7. <bbpart>BBa_K1172911</bbpart> - Biosafety-System L (lacI): Short part description.
8. <bbpart>BBa_K1172912</bbpart> - Part 1 of the Biosafety-System M (pRha TetR alr): Short part description.
9. <bbpart>BBa_K1172913</bbpart> - Part 2 of the Biosafety-System M with Barnase (TetO Barnase): Short part description.
10. <bbpart>BBa_K1172914</bbpart> - Part 2 of the Biosafety-System M with GFP (TetO GFP): Short part description.
11. <bbpart>BBa_K1172915</bbpart> - Biosafety-System M (TetR): Short part description.
12. <bbpart>BBa_K1172917</bbpart> - Double plac-Promoter with GFP: Short part description.