"
Team:Edinburgh/Introduction/Metal binding
From 2013.igem.org
| Line 8: | Line 8: | ||
We propose to make a system in which a metal binding protein is produced in response to the presence of that metal in the growth medium. As a proof of concept, we chose to work with iron. We planned to use the FUR box and protein to negatively regulate (in response to iron) an inverter gene which would then positively regulate expression of an iron binding protein. Thus the more iron that is present, the more iron binding protein is produced. | We propose to make a system in which a metal binding protein is produced in response to the presence of that metal in the growth medium. As a proof of concept, we chose to work with iron. We planned to use the FUR box and protein to negatively regulate (in response to iron) an inverter gene which would then positively regulate expression of an iron binding protein. Thus the more iron that is present, the more iron binding protein is produced. | ||
| - | We chose as our iron binding protein Ferric binding protein A (FbpA), from ''Neisseria gonorrhoeae''. It is a periplasmic protein and a member of the transferrin superfamily, which binds clusters of Fe3+ (Ferreiros ''et al.'' 1999; Alexeev ''et al.'' 2003) | + | We chose as our iron binding protein Ferric binding protein A (FbpA), from ''Neisseria gonorrhoeae''. It is a periplasmic protein and a member of the transferrin superfamily, which binds clusters of Fe3+ (Ferreiros ''et al.'' 1999; Alexeev ''et al.'' 2003). Predicted protein structure when bound to iron nanoparticles was presented on Figure 1. |
| + | |||
| + | [[File:structure.png]] | ||
<h2>References</h2> | <h2>References</h2> | ||
Revision as of 23:44, 4 October 2013
Contents |
Social channels
Follow us on
[http://www.facebook.com/Edigem2013 | Facebook] and
[http://twitter.com/EdiGEM2013 | Twitter]!
PDF project report
50pxWeb counter
Ferric binding protein A
We propose to make a system in which a metal binding protein is produced in response to the presence of that metal in the growth medium. As a proof of concept, we chose to work with iron. We planned to use the FUR box and protein to negatively regulate (in response to iron) an inverter gene which would then positively regulate expression of an iron binding protein. Thus the more iron that is present, the more iron binding protein is produced.
We chose as our iron binding protein Ferric binding protein A (FbpA), from Neisseria gonorrhoeae. It is a periplasmic protein and a member of the transferrin superfamily, which binds clusters of Fe3+ (Ferreiros et al. 1999; Alexeev et al. 2003). Predicted protein structure when bound to iron nanoparticles was presented on Figure 1.
References
Alexeev, D., Zhu, H., Guo, M., Zhong, W., Hunter, D., Yang, W., Campopiano, D. and Sadler, P. (2003) A novel protein-mineral interface. Nature Structural Biology. 10. 297-302.
Ferreiros, C., Criado, M. and Gomez, J. (1999) The Neisserial 37kDa ferric binding protein (FbpA). Comparative Biochemistry and Physiology Part B. 123. 1-7.
| 
| 
| 
| 
| 
|
| This iGEM team has been funded by the MSD Scottish Life Sciences Fund. The opinions expressed by this iGEM team are those of the team members and do not necessarily represent those of MSD | |||||
