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Team:Bielefeld-Germany/Labjournal/Molecular
From 2013.igem.org
(Difference between revisions)
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<col width="50"> | <col width="50"> | ||
<col width="50"> | <col width="50"> | ||
| - | <tr><th>Reagenz</th> <th>1x</th> <th>8</th> </tr> | + | <tr> <th>Reagenz</th> <th>1x</th> <th>8</th> </tr> |
| - | <td>Buffer 5x</td> <td>5,0</td> <td>44</td> | + | <tr> <td>Buffer 5x</td> <td>5,0</td> <td>44</td> </tr> |
| - | <td>MgSO4</td> | + | <tr> <td>MgSO4</td> <td>0</td> <td>0</td> </tr> |
| - | <td>dNTPs</td> <td>2,5</td> <td>22</td> | + | <tr> <td>dNTPs</td> <td>2,5</td> <td>22</td> </tr> |
| - | <td>Primer1</td> <td>1,0</td> <td>8,8</td> | + | <tr> <td>Primer1</td> <td>1,0</td> <td>8,8</td> </tr> |
| - | <td>Primer2</td> <td>1,0</td> <td>8,8</td> | + | <tr> <td>Primer2</td> <td>1,0</td> <td>8,8</td> </tr> |
| - | <td>Template</td> <td>1,5</td> <td>13,2</td> | + | <tr> <td>Template</td> <td>1,5</td> <td>13,2</td> </tr> |
| - | <td>DMSO</td> | + | <tr> <td>DMSO</td> <td>2,0</td> <td>17,6</td> </tr> |
| - | <td>Polymerase</td> <td>0,5</td> <td>4,4</td> | + | <tr> <td>Polymerase</td><td>0,5</td> <td>4,4</td> </tr> |
| - | <td>H2O</td> <td>11,5</td> <td>101,2</td> | + | <tr> <td>H2O</td> <td>11,5</td> <td>101,2</td> </tr> |
</table> | </table> | ||
</html> | </html> | ||
Revision as of 22:44, 6 August 2013
PCR Protocols and Programs
Phusion 25 µl
| Reagenz | 1x | 8 |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
