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Team:Bielefeld-Germany/Labjournal/Molecular
From 2013.igem.org
(Difference between revisions)
| Line 42: | Line 42: | ||
<tr><td>98°C</td><td>98°C</td><td>x°C</td><td>72°C</td><td>72°C</td></tr> | <tr><td>98°C</td><td>98°C</td><td>x°C</td><td>72°C</td><td>72°C</td></tr> | ||
<tr><td>2min</td> <td>30s</td><td>30s</td> <td>y</td><td>6min</td></tr> | <tr><td>2min</td> <td>30s</td><td>30s</td> <td>y</td><td>6min</td></tr> | ||
| - | <tr><td colspan="5">x: according to Primer Tm | + | <tr><td colspan="5">x: according to Primer Tm y: 15s/kb</td></tr> |
</table> | </table> | ||
</html> | </html> | ||
Revision as of 22:55, 6 August 2013
PCR Protocols and Programs
Phusion 25 µl
| Reagenz | 1x | 8x |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
| Program Phusion | ||||
|---|---|---|---|---|
| 98°C | 98°C | x°C | 72°C | 72°C |
| 2min | 30s | 30s | y | 6min |
| x: according to Primer Tm y: 15s/kb | ||||
