Team:Bielefeld-Germany/Labjournal/Molecular

From 2013.igem.org

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Line 42: Line 42:
<tr><td>98°C</td><td>98°C</td><td>x°C</td><td>72°C</td><td>72°C</td></tr>
<tr><td>98°C</td><td>98°C</td><td>x°C</td><td>72°C</td><td>72°C</td></tr>
<tr><td>2min</td> <td>30s</td><td>30s</td> <td>y</td><td>6min</td></tr>
<tr><td>2min</td> <td>30s</td><td>30s</td> <td>y</td><td>6min</td></tr>
-
<tr><td colspan="5">x: according to Primer Tm  &nbsp;&nbsp;&nbsp;  y: 15s/kb</td></tr>
+
<tr><td colspan="5">x: according to Primer Tm  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  y: 15s/kb</td></tr>
</table>
</table>
</html>
</html>

Revision as of 22:55, 6 August 2013




PCR Protocols and Programs

Phusion 25 µl

Reagenz 1x 8x
Buffer 5x 5,0 44
MgSO4 0 0
dNTPs 2,5 22
Primer1 1,0 8,8
Primer2 1,0 8,8
Template 1,5 13,2
DMSO 2,0 17,6
Polymerase0,5 4,4
H2O 11,5 101,2
Program Phusion
98°C98°Cx°C72°C72°C
2min 30s30s y6min
x: according to Primer Tm        y: 15s/kb

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