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From 2013.igem.org

Revision as of 22:58, 6 August 2013 (view source) Sgrenz (Talk | contribs) m ← Older edit		Revision as of 22:59, 6 August 2013 (view source) Sgrenz (Talk | contribs) Newer edit →
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	</table>		</table>
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		+
		+
		+
		+	<br><br>
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		+	<h1>PCR Protocols and Programs</h1>
		+
		+	<h3><a name="Taq">Taq 25 µl</h3>
		+
		+	<table border=1 style="float:left">
		+	<col width="100">
		+	<col width="50">
		+	<col width="50">
		+	<tr> <th>Reagenz</th> <th>1x</th> <th>8x</th> </tr>
		+	<tr> <td>Buffer 5x</td> <td>5,0</td> <td>44</td> </tr>
		+	<tr> <td>MgSO4</td> <td>0</td>      <td>0</td> </tr>
		+	<tr> <td>dNTPs</td>  <td>2,5</td> <td>22</td> </tr>
		+	<tr> <td>Primer1</td> <td>1,0</td> <td>8,8</td> </tr>
		+	<tr> <td>Primer2</td> <td>1,0</td> <td>8,8</td> </tr>
		+	<tr> <td>Template</td> <td>1,5</td> <td>13,2</td> </tr>
		+	<tr> <td>DMSO</td> <td>2,0</td>    <td>17,6</td> </tr>
		+	<tr> <td>Polymerase</td><td>0,5</td> <td>4,4</td> </tr>
		+	<tr> <td>H2O</td>    <td>11,5</td> <td>101,2</td> </tr>
		+
		+	</table>
		+
		+	<table border=1 style="float:left; margin-left:20px">
		+	<col width="50">
		+	<col width="50">
		+	<col width="50">
		+	<col width="50">
		+	<col width="50">
		+	<tr><th colspan="5">Program Phusion</th></tr>
		+	<tr><td>98 °C</td><td>98 °C</td><td>x °C</td><td>72 °C</td><td>72 °C</td></tr>
		+	<tr><td>2 min</td> <td>30 s</td><td>30 s</td> <td>y</td><td>6 min</td></tr>
		+	<tr><td colspan="5">x: according to Primer Tm <br> y: 15s/kb</tr>
		+
		+	</table>
		+
	</html>		</html>

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Revision as of 22:59, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Phusion
98 °C	98 °C	x °C	72 °C	72 °C
2 min	30 s	30 s	y	6 min
x: according to Primer Tm y: 15s/kb

PCR Protocols and Programs

Taq 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Phusion
98 °C	98 °C	x °C	72 °C	72 °C
2 min	30 s	30 s	y	6 min
x: according to Primer Tm y: 15s/kb