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Team:Bielefeld-Germany/Labjournal/Molecular
From 2013.igem.org
(Difference between revisions)
| Line 42: | Line 42: | ||
<tr><td>98 °C</td><td>98 °C</td><td>x °C</td><td>72 °C</td><td>72 °C</td></tr> | <tr><td>98 °C</td><td>98 °C</td><td>x °C</td><td>72 °C</td><td>72 °C</td></tr> | ||
<tr><td>2 min</td> <td>30 s</td><td>30 s</td> <td>y</td><td>6 min</td></tr> | <tr><td>2 min</td> <td>30 s</td><td>30 s</td> <td>y</td><td>6 min</td></tr> | ||
| - | <tr><td colspan="5">x: according to Primer Tm <br> y: 15s/kb | + | <tr><td colspan="5">x: according to Primer Tm <br> y: 15s/kb |
| - | + | ||
| - | <th>Notes</th> | + | <th>Notes</th> |
| - | < | + | </tr> |
</table> | </table> | ||
Revision as of 23:04, 6 August 2013
PCR Protocols and Programs
Phusion 25 µl
| Reagenz | 1x | 8x |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
| Program Phusion | |||||
|---|---|---|---|---|---|
| 98 °C | 98 °C | x °C | 72 °C | 72 °C | |
| 2 min | 30 s | 30 s | y | 6 min | |
| x: according to Primer Tm y: 15s/kb | Notes | ||||
Taq 25 µl
| Reagenz | 1x | 8x |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
| Program Taq | ||||
|---|---|---|---|---|
| 98 °C | 98 °C | x °C | 72 °C | 72 °C |
| 2 min | 30 s | 30 s | y | 6 min |
| x: according to Primer Tm y: 15s/kb | ||||
