Team:Paris Saclay/Notebook/August/1

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Revision as of 05:58, 12 August 2013

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Contents

Notebook : August 1

Lab work

o

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

1 - Gibson assembly.

  • BphR2 part1, BphR2 part2 and plasmid PSB1C3
  • _FNR_part1, FNR part1 and plasmid PSB1C3
  • RBS FNR part1 and FNR_part2 and plasmid PSB1C3

Protocol : PCR_clean_up


Adding amount:

  • Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 3 : Bbs FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)


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