Team:Paris Saclay/Notebook/August/1
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=='''Lab work'''== | =='''Lab work'''== | ||
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== | ||
===='''1 - Gibson assembly.'''==== | ===='''1 - Gibson assembly.'''==== | ||
- | * | + | * BphR2_part1, BphR2_part2 and plasmid PSB1C3 |
- | * | + | * FNR_part1, FNR part1 and plasmid PSB1C3 |
- | * | + | * RBS_FNR part1 and FNR_part2 and plasmid PSB1C3 |
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]] | Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]] | ||
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*Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) | *Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) | ||
*Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) | *Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) | ||
- | *Epperdorf 3 : | + | *Epperdorf 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) |
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{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} |
Revision as of 06:00, 12 August 2013
Contents |
Notebook : August 1
Lab work
A - Aerobic/Anaerobic regulation system / B - PCB sensing system
1 - Gibson assembly.
- BphR2_part1, BphR2_part2 and plasmid PSB1C3
- FNR_part1, FNR part1 and plasmid PSB1C3
- RBS_FNR part1 and FNR_part2 and plasmid PSB1C3
Protocol : PCR_clean_up
Adding amount:
|