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Team:Evry/Protocols/07
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<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | <p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | ||
PCR is divided into 5 steps:<br> | PCR is divided into 5 steps:<br> | ||
| - | First denaturation<br> | + | <ul><li>First denaturation<br> |
| - | Denaturation step<br> | + | <li>Denaturation step<br> |
| - | Anealing step<br> | + | <li>Anealing step<br> |
| - | Elongation step<br> | + | <li>Elongation step<br> |
| - | Final step<br> | + | <li>Final step<br></ul> |
The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p> | The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p> | ||
Revision as of 12:09, 21 August 2013
PCR and gel electrophoresis analysis
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
- First denaturation
- Denaturation step
- Anealing step
- Elongation step
- Final step
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
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