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Team:Evry/Protocols/02
From 2013.igem.org
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<h1> Protocol for transformation </h1> | <h1> Protocol for transformation </h1> | ||
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| + | <h2> Principle </h2> | ||
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| + | <h2> Preparation </h2> | ||
<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/> | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/> | ||
Let 30 minutes on ice.<br/> | Let 30 minutes on ice.<br/> | ||
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Let 5 minutes on ice.<br/> | Let 5 minutes on ice.<br/> | ||
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/> | Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/> | ||
| - | < | + | </p> |
| + | <h2> Preparation </h2> | ||
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Revision as of 10:46, 26 August 2013
Protocol for transformation
Principle
Preparation
For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.
Preparation
Site developped by Gabriel Guillocheau
