Team:Evry/Protocols/02

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<h1> Protocol for transformation </h1>
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<h1> Transformation </h1>
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<h2> Preparation </h2>
<h2> Preparation </h2>
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<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/>
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<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a
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<a href="https://2013.igem.org/Team:Evry/Protocols/05">Golden Gate</a> , add 5 µL instead.<br/>
Let 30 minutes on ice.<br/>
Let 30 minutes on ice.<br/>
Let the cells at 42°C for exactly 50 seconds.<br/>
Let the cells at 42°C for exactly 50 seconds.<br/>
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Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/>
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/>
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<h2> Preparation </h2>
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<h2> Tests</h2>
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Revision as of 10:51, 26 August 2013

Iron coli project

Transformation

Principle

Preparation

For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a Golden Gate , add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.

Tests

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