Team:Paris Saclay/Notebook/July/11
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{{Team:Paris_Saclay/incl_debut_generique}} | {{Team:Paris_Saclay/incl_debut_generique}} | ||
='''Notebook : July 11'''= | ='''Notebook : July 11'''= | ||
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+ | ===''Summary:''=== | ||
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+ | For régulation system: | ||
+ | *1.For those transformation products, RBS+LacS+terminator in PSB1C3 plasmid, the liquid culture has been performed for further experiments. The oligopetides for PCR amplification was made bioinformaticly. The transformation for the terminator BBa_B0010 was still fail. | ||
+ | *2. The plasmid which contains fnr+RBS+LacZ+terminator and fnr+RBS+AmilCP+terminator was extracted. The restiction digest was performer for them. | ||
+ | For sensor system: | ||
+ | *3. The selection of bacterian colonies was achieved by analyzing PCR product. The baterian selected are BphR1 c5 and c6; BphR2 c3 and c4; BphA1 c5 and c6,c7,c8. | ||
=='''Lab work'''== | =='''Lab work'''== |
Revision as of 02:58, 28 August 2013
Notebook : July 11
Summary:
For régulation system:
- 1.For those transformation products, RBS+LacS+terminator in PSB1C3 plasmid, the liquid culture has been performed for further experiments. The oligopetides for PCR amplification was made bioinformaticly. The transformation for the terminator BBa_B0010 was still fail.
- 2. The plasmid which contains fnr+RBS+LacZ+terminator and fnr+RBS+AmilCP+terminator was extracted. The restiction digest was performer for them.
For sensor system:
- 3. The selection of bacterian colonies was achieved by analyzing PCR product. The baterian selected are BphR1 c5 and c6; BphR2 c3 and c4; BphA1 c5 and c6,c7,c8.
Lab work
constructing
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