Team:Paris Saclay/Notebook/July/11

From 2013.igem.org

(Difference between revisions)
(Lab work)
(Notebook : July 11)
Line 1: Line 1:
{{Team:Paris_Saclay/incl_debut_generique}}
{{Team:Paris_Saclay/incl_debut_generique}}
='''Notebook : July 11'''=
='''Notebook : July 11'''=
 +
 +
 +
===''Summary:''===
 +
 +
For régulation system:
 +
*1.For those transformation products, RBS+LacS+terminator in PSB1C3 plasmid, the liquid culture has been performed for further experiments. The oligopetides for PCR amplification was made bioinformaticly. The transformation for the terminator BBa_B0010 was still fail.
 +
*2. The plasmid which contains fnr+RBS+LacZ+terminator and fnr+RBS+AmilCP+terminator was extracted. The restiction digest was performer for them.
 +
For sensor system:
 +
*3. The selection of bacterian colonies was achieved by analyzing PCR product. The baterian selected are BphR1 c5 and c6; BphR2 c3 and c4; BphA1 c5 and c6,c7,c8.
=='''Lab work'''==
=='''Lab work'''==

Revision as of 02:58, 28 August 2013

Notebook : July 11

Summary:

For régulation system:

  • 1.For those transformation products, RBS+LacS+terminator in PSB1C3 plasmid, the liquid culture has been performed for further experiments. The oligopetides for PCR amplification was made bioinformaticly. The transformation for the terminator BBa_B0010 was still fail.
  • 2. The plasmid which contains fnr+RBS+LacZ+terminator and fnr+RBS+AmilCP+terminator was extracted. The restiction digest was performer for them.

For sensor system:

  • 3. The selection of bacterian colonies was achieved by analyzing PCR product. The baterian selected are BphR1 c5 and c6; BphR2 c3 and c4; BphA1 c5 and c6,c7,c8.

Lab work

constructing



Previous day Back to calendar Next day