Team:Macquarie Australia/Protocols/PCR

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Revision as of 04:46, 29 August 2013


Polymerase Chain Reaction


PCR mixture
4.0ul 5x phusion buffer 0.4ul dNTPs 0.6ul DMSO 0.2ul Polymerase 11.8ul water Total 17ul 1ul forward primer 1ul reverse primer 1ul template

PCR settings
Initial denaturation 98oC for 30 secs Followed by 30 repears of: Denaturation 98 oC 10 secs Annealing 60 oC 10 secs Extension 72 oC 2 mins Final extension 72 oC 10 mins

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