Team:TU-Delft/Notebook/2013/08/22/

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<p> 1. pBAD:Agr AC:pP2 <br>  
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<p> 1. pBAD:Agr AC:pP2, restriction digested back bone of <i> B. subtilis </i>, AIP:GFP:TT was run on gel electrophoresis.  <br>  
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2. Carried out colony PCR on His-SUMO peptides with correct primer for pET23B and ran on gel. This gel gave good results. <br>
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2. Inoculation of pBAD(BBa_K20600) from glycerol stock in LB broth with chloroamphenicol resistance. <br>
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3. Inoculation of pTET:RBS:TT.  <br>
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4. Sent samples of pBAD:AgrAC:pP2 from colony 3 and colony 7 for sequencing. <br>
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3. Transformation of pBAD:AgrAC:pP2 was done in XL1 Blue cells and plated on agar plates.  <br>
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4. PCR purification of His-SUMO-Peptides was done. <br>
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5. 10X TBE buffer was made and kept in stock. <br>
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Revision as of 20:52, 31 August 2013

Notebook

June
SunMonTueWedThuFriSat
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
SunMonTueWedThuFriSat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31










August
SunMonTueWedThuFriSat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
SunMonTueWedThuFriSat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30












22nd of August


Lab work

1. pBAD:Agr AC:pP2, restriction digested back bone of B. subtilis , AIP:GFP:TT was run on gel electrophoresis.




2. Inoculation of pBAD(BBa_K20600) from glycerol stock in LB broth with chloroamphenicol resistance.
3. Inoculation of pTET:RBS:TT.
4. Sent samples of pBAD:AgrAC:pP2 from colony 3 and colony 7 for sequencing.

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