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User contributions
From 2013.igem.org
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- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:09, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:07, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:05, 23 August 2013 (diff | hist) N 23/08/13 (Created page with "==Nanodropping overnight incubations of herring sperm== *Table representing the concentrations of herring sperm after and before different treatments {|border=1 |Sample||Concentr...")
- 09:19, 23 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:07, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 09:03, 23 August 2013 (diff | hist) N File:Igem shear son 220813.jpg (top)
- 09:03, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 12:42, 21 August 2013 (diff | hist) Team:Leicester/Notebook
- 12:42, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) N 20/08/13 (Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...")
- 14:16, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 13:58, 19 August 2013 (diff | hist) N File:Igem denatured son 190813.jpg (top)
- 13:57, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) N 19/08/13 (Created page with "==Denaturing sonicated DNA== *Denaturing DNA by heat shock for 10mins in a 95C heat block *Adding 5ul of OG dye ==Running both denatured and sonicated samples on gel== *For just...")
- 11:01, 19 August 2013 (diff | hist) Team:Leicester/Notebook
- 13:40, 16 August 2013 (diff | hist) 16/08/13 (→Running the gel from sonication series)
- 13:39, 16 August 2013 (diff | hist) N File:Igem 2nd son series 160813.jpg (top)
- 13:39, 16 August 2013 (diff | hist) N 16/08/13 (Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
- 13:34, 16 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:20, 16 August 2013 (diff | hist) 18/07/13 (Replaced content with "==Double digest==")
- 12:01, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:50, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:50, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:49, 12 August 2013 (diff | hist) 12/08/13 (→Sonicating herring sperm DNA)
- 11:49, 12 August 2013 (diff | hist) N 12/08/13 (Created page with "==Sonicating herring sperm DNA== *Pipetting out sonicated samples every 30mins **Pipette 4ul of DNA into Son 1-6 every 30mins ***Dilute 1ul from Son 1-6 in 9ul of 1xTE buffer to ...")
- 11:34, 12 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:30, 12 August 2013 (diff | hist) 09/08/13 (→Glycerol stocks)
- 09:29, 12 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 16:33, 9 August 2013 (diff | hist) N File:Lim chlorodig sacI 090813.jpg (top)
- 16:33, 9 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 16:00, 9 August 2013 (diff | hist) 09/08/13 (→Sonicating Herring sperm DNA)
- 15:28, 9 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 10:15, 7 August 2013 (diff | hist) 07/08/13
- 10:06, 7 August 2013 (diff | hist) N 07/08/13 (Created page with "{|border=1 |Sample||Concentration||260/280||260/230 |- |1kb||534||1.82||1.95 |- |1||52.7||1.77||2.05 |- |2||4.6||1.46||1.88 |- |3||0.1||0.24||-0.29 |- |Overnight |- |1in 10||24.8...")
- 08:35, 7 August 2013 (diff | hist) Team:Leicester/Notebook
- 10:03, 6 August 2013 (diff | hist) 06/08/13 (→Running of an agarose gel)
- 16:30, 31 July 2013 (diff | hist) Team:Leicester
- 15:41, 31 July 2013 (diff | hist) N File:Lim1 digest 31 07 2013.jpg (The bands are well separated as shown by the middle 4 lanes, the two outermost lanes are the markers. 5um of 1kb ladder was used as a marker for each of the two lanes and 10um of orange G loading dye was added to the samples.) (top)
- 15:37, 31 July 2013 (diff | hist) 31/07/13 (→Results from the Transformation of limonene Biobrick (BBa_K118025) to the pSB1C3 vector)
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