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Team:Evry/Protocols/15
From 2013.igem.org
Gel electrophoresis analysis
Principle
After PCR, electrophoresis is a method using electrical field to separate DNA or RNA sequences by size. Smaller the fragment is, the more it migrates on the gel.
Using a DNA ladder, we can know the size of DNA sequence and then check if we have the sequence we wanted.
Preparation
Agarose gel 1% preparation:
Add 0,5 g of Agarose in 50 mL of TAE 1X
(See protocole for TAE 1X preparation there)
Put the solution into microwave until Agarose is disolved.
When until the cool down and add 3 mL of Ethidium Bromide (EtBr).
Put the solution in the cuve and let it cool down.
Mix gel
Put 5 μL of PCR product and 1 μL of loading dye 6X in each well.Do not forget your positive and negative controles, and the kB ladder. Put in the electrophoresis cell at 100 V during 40 minutes.
Analysis
Site developped by Gabriel Guillocheau
