Team:Paris Saclay/Notebook/August/29
From 2013.igem.org
Notebook : August 29
summary
- We got clonies on ligation promoter fnr(activator)nirB plus RBS_LacZ+Term_PSB1C3
promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3 promoter fnr(repressor) plus RBS_LacZ+Term_PSB1C3 promoter fnr(repressor) plus RBS_AmilCP+Term_PSB1C3t so we do Colony PCR for it
- We didn't get clonies on Gibson so we do a gel electrophoresis of parts of Gibson assembly to verify the size
- Digestion Dnp1 purification of the the PSB1C3 clean for Gibson and electrophoresis of it .
lab work
- A.aero/anaerobic regulation system
- Digestion for PSB3K3
DNA | 9µl |
Ecoil I | 2µl |
PST I | 2µl |
H2O | 5µl |
buffer | 2µl |
total | 20µl |
- Digestion for RBS_LacZ+Term_10(08/09/13)
B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3
DNA | 14µl |
xpe I | 2µl |
PST I | 2µl |
buffer | 2µl |
total | 20µl |
- Digestion for RBS_LacZ+Term_11(08/09/13)
B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3
DNA | 14µl |
xpe I | 2µl |
PST I | 2µl |
buffer | 2µl |
total | 20µl |
- Digestion for RBS_LacZ+Term_15(08/09/13)
B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3
DNA | 14µl |
xpe I | 2µl |
PST I | 2µl |
buffer | 2µl |
total | 20µl |
- Digestion for RBS_AmilCP+Term_9(02/09/13)
DNA | 14µl |
xpe I | 2µl |
PST I | 2µl |
buffer | 2µl |
total | 20µl |
- Digestion for RBS_AmilCP+Term_12(02/09/13)
DNA | 14µl |
xpe I | 2µl |
PST I | 2µl |
buffer | 2µl |
total | 20µl |